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作 者 ：张富铁;庄延;杨之帆;杨海元;祝莉莉;何光存

期 刊 ：武汉植物学研究 2005年 23卷 1期 页码：1-6

关键词：褐飞虱；白背飞虱；药用野生稻；cDNA-AFLP；应答；

Keywords:Brown planthopper (Nilaparvata lugens (Stå, l)), Whitebacked planthopper (Sogatella furcifera Horvath), Oryza officinalis Wall ex Watt, cDNA-AFLP, Response,

摘 要 ：褐飞虱和白背飞虱是水稻的刺吸式害虫。为了探讨水稻对虫害反应的分子机理,采用cDNA-AFLP(互补DNA扩增片段长度多态性)技术,研究了药用野生稻受这两种飞虱取食后基因表达的变化。通过调查约7%的虫应答后的药用野生稻转录本组,共分离了258个差异片段,测序112个;44个片段对应于已知或假定功能基因,其中35个基因的表达水平在白背飞虱为害后发生改变,24个基因在褐飞虱为害后改变。下调的基因比上调的基因多。有14个基因受这两种飞虱共同调节(2个上调,12个下调)。结果表明,药用野生稻对两种飞虱为害作出的反应有所不同。

Abstract:Brown planthopper (Nilaparvata lugens (Stål),BPH) and whitebacked planthopper (Sogatella furcifera Horvath,WBPH) are the phloem-sucking herbivores of rice.We employed cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique to characterize the mole-(cular) responses of wild rice Oryza officinalis Wall ex Watt to BPH and WBPH feeding.After investigating about 7% of the insects-responsive transcriptome,we isolated 258 differential transcript-derived fragments (TDFs) and sequenced 112,of which 44 presented similarities to known or putative functional proteins.Among these 44 TDFs,35 TDFs were altered by WBPH-feeding while 24 were by BPH-feeding.There were more down-regulated TDFs than the up-regulated ones found after fed by BPH and WBPH.Only 2 TDFs were co-induced but 12 were co-suppressed by two planthoppers feeding.The results revealed that O.officinalis responded differentially to these two phloem herbivores.

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