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作 者 ：何丹;徐跃进;李正丽;汪祖程

期 刊 ：武汉植物学研究 2009年 27卷 2期 页码：221-224

关键词：甘蓝型油菜；红菜薹；远缘杂交；子房培养；

Keywords:Brassica napus, Purple-caitai, Distant hybridization, Ovary culture,

摘 要 ：对甘蓝型油菜和红菜薹种间杂种进行胚胎挽救研究,实验结果表明,采用MS+(1.0～2.0)mg·L^-16-BA+0.05mg·L^-1NAA+0.5%活性炭+30g·L^-1蔗糖+7.5g·L^-1琼脂培养基对甘蓝型油菜和红菜薹杂交子房培养效果较为理想;相对于培养基和激素,活性炭对子房培养的影响更加显著。通过对取材时间的研究发现,取授粉后18d子房培养的结籽率最高,15d的次之。而通过对杂种萌发率的研究则表明,授粉后15d的子房培养获得的杂种萌发率最高,为57.03%,18d的最差,仅为38.49%。

Abstract:The embryo rescue technique was applied to obtain interspecific hybrids between Brassica napus and Purple-caitai.Of the media studied,MS supplemented with(1.0-2.0)mg·L^-1 6-BA+0.05 mg·L^-1 NAA+0.5% activated carbon+30 g·L^-1 sugar+7.5 g·L^-1 agar gave the most satisfactory results for ovary culture.The effect of activated carbon is more significant than that of medium and plant hormone in ovary culture.Ovaries taken 9,12,15 and 18 days after pollination were used for culture,and those taken 18 days after pollination appeared most efficient for seed production,that of 15 days takes second place.But the results by studying the germination of their hybrid seeds showed the germination rate of the ovary cultured hybrids taken 15 days after pollination is highest,namely 57.03%,the lowest in 18 days,Only 38.49%.

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