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作 者 ：樊明琴;朱月林;朱丽华

期 刊 ：武汉植物学研究 2005年 23卷 2期 页码：138-143

关键词：结球白菜；不定芽再生；组织学变化；生理变化；

Keywords:Brassica rapassp.pekinensis(L.)Olsson, Adventitious shoot regeneration, Histological change, Physiological change,

摘 要 ：以日本引进品种爱知结球白菜(Brassicarapa ssp.pekinensiscv.AichiHakusai,C₁)为试材,对离体子叶不定芽再生过程中的组织学和生理变化进行了研究。结果表明,子叶在离体培养过程中,不定芽发生方式为器官直接发生。在不定芽形成前,可溶性蛋白质含量、POD和SOD的活性均呈上升趋势。随着细胞的脱分化,代谢活动逐渐旺盛,酶活性增强,可溶性蛋白质含量增加,表明不定芽形成过程中形态变化与生理变化紧密相联。培养基中添加AgNO₃对酶活性有促进作用,并促进不定芽的分化。

Abstract:Aichi Hakusai(C₁),which was a Chinese cabbage cultivar(Brassicarapa ssp.pekinensis(L.)Olsson)introduced from Japan,was used as plant material in this experiment.Investigations were carried out on histological and physiological changes during the process of in vitro shoot regeneration from cotyledonary explants.Histological observations showed that,(adventitious)shoots were induced through the pathway of direct organogenesis during the process of explants culture.Before adventitious shoots initiated,soluble protein content and activities of POD and SOD showed the trend to increase.As the cells were dedifferentiated,metabolism activity of cells gradually became stronger;enzymes activity and the soluble protein content were elevated.These results demonstrated that,morphological changes were strongly associated with physiological changes during the process of adventitious shoot induction.Addition of AgNO₃ to the medium increased the activity of enzymes and promoted the differentiation percentage of(adventitious)shoots.

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