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作 者 ：顾福根;孙丙耀;韵宇飞;练兆云

期 刊 ：武汉植物学研究 2008年 26卷 6期 页码：639-643

关键词：石龙尾；茎段；组织培养；快速繁殖；间歇灭菌；

Keywords:Limnophila sessiliflora BI., Stem segment, Tissue culture, Rapid propagation, Intermittent sterilization,

摘 要 ：以石龙尾(Limnophila sessiliflora BI.)沉水枝带节茎段为外植体进行离体培养,研究外植体灭菌方法以及培养基中不同生长调节剂的浓度对其增殖、生根的影响。结果表明:以0.1%的HgCl2为灭菌剂,采用4 min+4 min、间歇4 h的间歇灭菌法,可以获得成活的无菌外植体15%;在1/2 MS+6-BA 2.0 mg·L^-1+NAA 0.1~0.2 mg·L^-1的增殖培养基上培养35 d,试管苗的增殖系数可达30.8以上;在1/2 MS+6-BA 0.3 mg·L^-1+NAA0.5 mg·L^-1的生根培养基上培养28 d后,可获得具3~5个侧枝的生根苗,平均每株生根数4.8条;炼苗后移植成活率100%。

Abstract:The in vitro culture of Limnophila sessiliflora was established by using the nodal stem segments from submerged branch as explants.The method of surface sterilization of explants,the effects of different plant growth regulators on the multiplication and rooting were investigated.The results showed that 15% sterile explants could be obtained by twice surface treatment with 0.1% HgCl2 for 4 min with an interval of 4 h.When cultured on a 1/2 MS multiplication medium supplemented with 2.0 mg·L^-1 of 6-BA and 0.1-0.2 mg·L^-1 of NAA for 35 d,the test-tube seedling exhibited a multiplication coefficient higher than 30.8.For optimal rooting,induction on a 1/2 MS rooting medium supplemented with 0.3 mg·L^-1 of 6-BA and 0.5 mg·L^-1 of NAA for 28 d could produce rooted plantlets with 3-5 lateral shoots,and each plantlet generated 4.8 roots on average.Rooted plantlets were acclimatized and transferred into outdoor aquatic environment with 100% survival ratio.

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