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作 者 ：张宜麟;赵帆;赵洁

期 刊 ：武汉植物学研究 2005年 23卷 3期 页码：203-210

关键词：水稻；种子萌发；脱落酸；OsMAPK5；BGLU2；OsCKI1；

Keywords:OryzasativaL., Seedgermination, ABA, OsMAPK5, BGLU2, OsCKI1,

摘 要 ：植物激素脱落酸(abscisicacid,ABA)调控着植物发育过程中的许多重要事件。实验以水稻(嘉育948)种子为材料,通过3种不同的外源ABA处理方法,观察萌发过程中根和芽的生长变化。结果表明,外源ABA对水稻种子的萌发率没有明显的影响,而对种子萌发过程中根和芽的生长速率具有显著的抑制作用(1A3A和1N3A);外源ABA对种子萌发过程中的抑制作用可部分逆转和恢复(1A3N);种子在低浓度的ABA中浸泡1d后再萌发,对根的生长有轻微的促进(1A3N)。通过RT-PCR和Northern杂交分析了水稻促有丝分裂原蛋白激酶5(OsMAPK5)、水稻酪蛋白激酶1(OsCKI1)和β-葡糖苷酶2(BGLU2)3个基因在水稻种子萌发过程中(1A3A)转录水平上的表达差异,发现ABA抑制了这3个基因的表达,同时也影响了它们在根和芽中的表达模式。

Abstract:Many important aspects during plant development are regulated by abscisic acid (ABA).In this study,the seeds from rice variety “Jiayu 948”(Oryza sativa L.) were germinated in ABA solution using three kind methods of ABA treatments.The morphological characteristics of the seeding growth were observed.The results showed that exogenous ABA had affects on the frequency of seed germination and inhibited the growth rate of rice seed obviously (1A3A and 1N3A),but the effects of the inhibition were partially reversible (1A3N).Low concentration of ABA could induce the root growth slightly (1A3N).Furthermore,the expression patterns of three genes were analyzed,O.sativa Mitogen -activated protein kinase 5 (OsMAPK5),β-glucosidase2 (BGLU2) and Oryza sativa Casein kinaseⅠ1 (OsCKI1) during the germination of rice seeds(1A3A).We found that the expressions of OsMAPK5,OsCKI1 and BGLU2 were down-regulated along with the increasing concentration of ABA and changed in root and shoot.

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