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作 者 ：马剑敏;蔡林林;靳同霞;李效宇

期 刊 ：武汉植物学研究 2008年 26卷 5期 页码：509-513

关键词：离子液体；斜生栅藻；超氧化物歧化酶(SOD)；过氧化氢酶(CAT)；丙二醛(MDA)；

Keywords:Ionic liquid, Scenedesmus obliquus, SOD, CAT, MDA,

摘 要 ：研究了1、5、10、15、20mg·L^-1的离子液体溴化1-己基-3-甲基咪唑([C₆mim]Br)在处理斜生栅藻24、48、96h时对其抗氧化酶系统和膜脂过氧化的影响。结果表明:[C₆mim]Br处理24h时,在各浓度水平均引起藻细胞可溶性蛋白和丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的显著增高。48、96h时,SOD活性在各浓度水平均维持在较高水平,最大值出现在较低处理浓度下;其他指标有随着处理浓度增加而升高的趋势,且显著高于对照,但其数值多数比24h时的有所下降。CAT活性与可溶性蛋白含量的变化趋势很相似,两者与MDA含量的变化趋势也较相似。[C₆mim]Br造成了藻细胞的明显脂质过氧化反应,具有一定的环境毒性;抗氧化酶SOD和CAT可以作为[C₆mim]Br胁迫分子水平上的敏感生物标志物。

Abstract:The effects of ionic liquid 1-hexyl-3-methylimidazolium ([C₆mim]Br) at the concentrations of 1,5,10,15 and 20 mg·L^-1 on antioxidant enzyme activities,lipid peroxidation of Scenedesmus obliquus were determined in the present study after treated for 24,48 and 96 hours,respectively.The results indicate:After exposed for 24 hours in [C₆mim]Br,the contents of soluble protein and malondialdehyde (MDA),the activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly at all the concentrations.At the 48th hour and 96th hour SOD activities were at higher level.Its peak values appeared at the lower concentration of [C₆mim]Br.The change trends of the activities of CAT,the contents of soluble protein and MDA were increasing with the increasing of the concentration of [C₆mim]Br at the 48th hour and 96th hour,but compared with the data at the 24th hour,most of them decreased a little.The change trends of the CAT activities had great similarities with that of the contents of soluble protein.Both their change trends were also similar to that of MDA content.The [C₆mim]Br induced the lipid peroxidation of S.obliquus and had environmental toxicity.SOD and CAT could be used as sensitive biomarkers at the molecule level of the stress of [C₆mim]Br.

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