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作 者 ：葛茜, 邝静, 武玉翠, 张媛, 肖娅萍, 王喆之

期 刊 ：植物科学学报 2013年 31卷 3期 页码：261-268

关键词：丹参；肉桂醇脱氢酶；生物信息学分析；基因表达；

Keywords:Salvia miltiorrhiza Bunge, Cinnamyl alcohol dehydrogenase, Bioinformatics analysis, Gene expression,

摘 要 ：肉桂醇脱氢酶(cinnamyl alcohol dehydrogenase, CAD)依赖于NADPH还原肉桂醛及其衍生物,是催化木质素单体生物合成途径的最后一步关键酶。通过分析丹参转录组数据库,从丹参中获得一条肉桂醇脱氢酶基因,命名为SmCAD (Genebank注册号:HQ162287)。该序列包含一个长为1083 bp的开放阅读框,有3个内含子和4个外显子,编码360个氨基酸,含有NADP(H)结合域,Zn1和Zn2锌结合位点。利用BD walking的方法获得其启动子序列1202 bp,序列分析结果表明,SmCAD启动子区包含茉莉酸甲酯(MeJA)、脱落酸(ABA)、赤霉素(GA₃)响应元件以及MYB结合位点。利用实时荧光定量PCR分析表明,该基因在丹参根、茎、叶中均有表达,且其表达受到MeJA的诱导和GA₃的抑制,推测该基因可能参与了丹参对外源信号的应答反应。研究结果可为进一步研究SmCAD基因在丹参中的具体功能提供理论依据。

Abstract:Cinnamyl alcohol dehydrogenase (CAD) catalyzes the reduction reaction of cinnamic aldehyde and its derivatives depending on the NADPH.It is the key enzyme in the last step of lignin biosynthesis. A novel CAD gene designated as SmCAD (GenBank accession No.: HQ162287) was cloned from Salvia miltiorrhiza Bunge according to the transcription databases. It contained a 1083 bp open reading frame, including 3 introns and 4 exons, and encoded a protein of 360 amino acids. The predicted SmCAD protein had Zn1 and Zn2 binding sites and NADP(H) binding domain. A total of 1202 bp promoter sequences were obtained using BD walking method, and sequence analysis revealed that there were MeJA, ABA, GA₃ response elements and MYB binding sites. Real-time quantitative PCR showed that SmCAD was expressed in different organs, and its expression was induced by MeJA and inhibited by GA₃. These results suggested that this gene may be involved in the response of exogenous signals. This study contributes to the theoretical basis of the specific function of SmCAD.

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