Abstract:The chromosome number and karyotype of seven species of Indigofera L.were studied by applying the root tip squash technique.Results show that six species were diploid(2n= 2x=16),while Indigofera zollingeriana Miq.was tetraploid(2n= 2x= 32).The karyotypes of I.zollingeriana Miq.and I.silvestrii Pamp.belonged to 2A,while the others were 1A.The karyological difference between species was small.Most chromosomes in the tested seven species were metacentric.The karyomorphology of I.delavayi Franch.,I.scabrida Dunn,I.silvestrii Pamp.,I.szechuensis Craib and I.zollingeriana Miq.are reported here for the first time.