---

作 者 ：刘文芳;吴文瑜;肖翊华

期 刊 ：武汉植物学研究 1987年 5卷 3期 页码：267-274

关键词：酯酶同工酶；杂交水稻；互补型酶谱；杂种优势预测；器官特异性；

Keywords:Hybrid rice, Esterase isozyme, Complementary zymogram, Hybrid vigor prediction, Organ speciality,

摘 要 ：本文报道了利用聚丙烯酰胺凝胶电泳技术,测定杂交水稻及三系亲本共50个组合的萌动胚、芽,不同发育时期的叶片、根、雄蕊等12个组织或器官的酯酶同工酶的结果。根据所测结果,可把杂交水稻的酯酶同工酶酶谱分为5种类型:互补型、偏父型、偏母型、同型和"杂种"酶谱。强优势组合以互补型酶谱居多,弱优势组合都是同型酶谱,"杂种"酶谱仅见于V优64的幼穗分化期叶片和V优63、汕优63的三叶期叶片中。不同器官的互补酶谱都可作为预测杂种比势,鉴定杂交稻种子的纯度和真实性以及选配新杂交组合的一个手段或依据,但以对萌动胚或幼芽的测定更有实践意义。

Abstract:In this paper, it is reported that the results of determining esterase isozymes of 12 various tissues or organs of embryo, germinate, leaves, stamen ctc. from 50 hybrid rice and their parents by means of polyacrylamide gel electrophoresis. According to our experiment, the zymograms of csterase isozymes of hybrid rice can be divided into 5 types: complementary, patroclinal, matroclinal, homotypic and "hybrid" zymograms. In stronger hybrid vigor combination, there are more complementary zymogram and less patroclinal, matroclinal and homotypic zymograms. In weaker hybrid vigor combination, there are only homotypic zymogram. "Hybrid" zymogram only cxists in leaves at three-leaf stage of both Shan You 63 and Wei You 63 and leaves at differentiation stage of young panicle of Wei You 64.

---