摘 要 ：本实验利用云南永胜县程海湖的碱水配制培养基,在当地的气候条件下驯化培养钝顶螺旋藻(Spirulina platensis),筛选得CH-1,CH-2和CH-3三种培养基配方。室外连续培养共240天,其中采用CH-2培养基室外大量培养90天,培养面积75平方米,连续测定20天的干藻粉产量为12.5克/米2·天,纯度为95%,干藻粉的蛋白质含量55—63%。除色氨酸未测定外,含有另外的17种氨基酸。从程海碱水驯化培养的钝顶螺旋藻群体中,筛选获得一个螺旋藻新品系——Spirulinaplatensis str. CH-1。与原品系比较,其特点是藻丝和螺距都较长,螺旋更宽,具有上浮性较好的优势,适合在用程海碱水配制的培养基中生长。实验结果表明,利用程海丰富的湖水资源和适宜的气候条件,发展螺旋藻生产是可行的。
Abstract:Lake Chenghai is located 1503m above sea level on Yun Gui Plateaus in China. It belongs to subtropics semidryness climate, annual average temperature is 19℃, rainfall averages about 740mm per year. Lake Chenghai is deeper with an average depth of 25.7m. The lake area is 77.2km2. It‘s an alkaline lake, the water pH ranges from 8.6 to 9.3. The cultivation of Spirulina platensis in the water of Lake Chenghai has been studied under the climate conditions of Chenghai region. CH-1, CH-2 and CH-3 media have been got in this experiment. The outdoor culture lasted 240 days. The cultivation area is 75m2. Dried algae was 12.5/m2.d in 20 days. The purity is up to 95%. The protein contents ranged from 55% to 63% of the dry weight. Except TRP which has not been measured, it contained another 17 kinds of amino acids, From the population of Spirulina platensis which had been cultivated in the water of Lake Chenghai, a new strain, Spirulina platensis str. CH-1, was selected. To compare with eriginal strain, its algal filament and thread pitch are longer, and the helix is also wider. It has clear superiority of floating character. It suits to growth in the media that made up with alkalic water.Our experiment show that it is suitable to develop Spirulina production in Chenghai region.