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作 者 ：柯善强;陈海芳;黄仁煌;武显维;王圣梅

期 刊 ：武汉植物学研究 1989年 7卷 1期 页码：59-64

关键词：叶外植体；不定芽；同工酶；细胞分化；

Keywords:tissue culture, blackberry, Rubus, cellular differentiation, brambles.,

摘 要 ：本文在裂叶悬钩子的叶外植体培养不定芽发生的基础上,利用圆盘聚丙烯酰胺凝胶电泳技术,研究了其细胞分化过程中过氧化物酶同工酶的变化。实验指出:在有6-BA或2.4-D与NAA组合的培养条件下,随着培养时间和分化进程不同,过氧化物酶同工酶有变化,谱带由4条增加到8条,酶的活力分别由△0.24 OD/min提高到△1.82OD/min和△1.90 OD/min。在无外源激素条件下,过氧化物酶同工酶的变化不大,酶的活力也低,因而,叶外植体组织细胞也不能分化成植株。本文还从分子水平上探讨了酶和基因与细胞分化的关系。

Abstract:Changes in levels of peroxidase isozymes during in vitro adventitious bud formation from leaf explants of Rubus laciniatus wild. were investigated by means of disc acrylamide gel electrophoresis. The overall level of peroxidase isozymes increased four to eight times and the enzyme activity increased from 0.24 OD/min to 1.82 OD/min during the process of differentiation in vitro. In addition, various growth regulators had different effects on the level of peroxidase during adventitious shoot formation. Without growth regulators, there was little change in the level of peroxidase isozymes and no shoot differentiation. The relationship of enzyme activity to gene activity and cellular differentiation is discussed in this paper.

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