田间栽培和植物生长室培养试验结果表明，净光合速率（Pn）在尚未形成生殖器官的一年生人参叶片完全展开后即达最大值，此后缓慢下降；2~6年生人参叶片完全展开后达第1个高峰，开花期略有下降，绿果期出现第2高峰，此后持续下降。去掉花蕾的人参植株叶片在对照植株的绿果期没有出现第2高峰,但在红果期和黄叶期净光合速率下降缓慢。弱光（10%透光荫棚）和适宜光（30%透光荫棚）下人参叶片绿果期后Pn下降缓慢，强光（50%透光荫棚）下下降较快，过早出现变黄早衰。强光和高温可使植株生育期缩短、叶片早衰、Pn快速下降，而弱光和低温使植株生育期延长，Pn下降时间推迟。叶片表观量子效率(AQY)、气孔导度(Gs)和蒸腾作用(Tr)自展叶期至绿果期变化不大，红果期和黄叶期持续下降。胞间CO2浓度(Ci)在展叶期至绿果期较低，红果期和黄叶期持续增加，说明生育后期Pn下降是由非气孔限制因子引起。叶片Pn与比叶重呈负相关，推测叶片光合产物的积累和消耗与Pn的生育期变化有关。绿果期Ci最低，同时水分利用效率(WUE)较高，是人参叶片光合作用对水分需求的关键时期。
Panax ginseng is a famous special herb medicine. As a perennial shade-loving medicine crop, it will be harvested after growing at least 5–6 years. The net photosynthetic rate(Pn) is very low (2–4 µmol CO2·m-2·s-1) in leaves of Panax ginseng , which is an important limitation factor for production. In order to investigate the changes of the characteristics and the limitation factors of photosynthesis in Panax ginseng leaves at different growth stages, net photosynthetic rate (Pn) and related parameters in leaves of 1–6 years old Panax ginseng grown in the field and in phytotron were measured by infrared gas analyzer. The net photosynthetic rate reached the maximum , then decreased with leaf age in fully expanded leaves of one-year old Panax ginseng without forming reproductive organ. In leaves of 2–6 years old plant, the first peak of net photosynthetic rate appeared in the fully expanded leaves, then Pn had little decrease at flowering stages and reached the second peak at green fruit stage (fruit growing stage), at last persistently decreased at red fruit(ripe fruit) and yellowing leaf stages. For the experiment with removing flower bud, the second Pn peak did not appeared at green fruits, and Pn was higher in red fruits and yellowing leaf stages, as compared with control plants. The results showed that the changes of Pn are regulated by reproductive growth at different growth stages. Apparent quantum yield (AQY) did not show obvious changes from leaf fully expanded to green fruit stages, which the maximum AQY was observed in fully expanded leaves. AQY was decreased with the decrease of Pn after green fruit stage. The leaves grown under low light (10% of full light) and optimum light (30% of full light) were showed more slowly decrease of Pn than these under high light (50% of full light) after green fruit stage. The leaf senescence and the decrease of Pn were accelerated in each growth stage under high light and high temperature; conversely the senescence and the decrease of Pn were delayed in each growth stage under low light and low temperature. Stomatal conductance(Gs) and transpiration rate(Tr) had similar change trends to Pn at different growth stages. Intercellular CO2 concentration(Ci) was kept stable from leaf fully expanded to green fruit, and increased persistently after green fruit. The changes of Ci indicated that the decrease of photosynthesis was not caused by stomatal limitation at red fruit and yellowing leaf stages. There was negative correlation between Pn and specific leaf weight (SLW) from leaf fully expanded to red fruit. So, it can be deduced that changes of photosynthesis are related with accumulation and demand of photosynthate. There were higher WUE and lower Ci at green fruit then at other stages. The results indicate that water supply at green fruit stage is very important to improve the photosynthetic productivity.
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