全 文 ： ACTA AGRONOMICA SINICA 2008, 34(1): 163−166 http://www.chinacrops.org/zwxb/
ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@chinajournal.net.cn

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Received(CDEF): 2007-01-29; Accepted(GHEFI: 2007-04-24.
DOI: 10.3724/SP.J.1006.2008.00163
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Screening and Analysis of Resistance to Silique Shattering in Rape (Brassica
napus L.)
WEN Yan-Cheng1, 2, FU Ting-Dong1,*, TU Jin-Xing1, MA Chao-Zhi1, SHEN Jin-Xiong1,
and ZHANG Shu-Fen2
( 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei; 2 Institute of Industrial
Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China)
Abstract: The silique shattering resistance index (SSRI) of 229 accessions (Brassica napus L.) was investigated through random
impact test (RIT) for screening silique shattering (SS) resistance. The accessions displayed wide variation in SSRI, which ranged
from 0.000 to 0.7675 with the variance coefficient (CV) of 114.4%. Only two varieties (accounting for 0.9%) showed SS resis-
tance; about 3.93% of the varieties (lines) showed relative resistance to SS; and about 8.73% were identified as the medium. Most
(59.38%) of the varieties (lines) were identified as very susceptible germplasm to SS, and 27.07% as the susceptible germplasm.
According to independent experiments in three years with 6 representative varieties (lines), the SSRI was dominated by genetic
factor with obvious interactions with environment, and the RIT showed stable results within years. The simple correlation analysis
showed that the SSRI had significant (P<0.01) negative correlation with silique number per centimeter, and significant (P<0.01)
positive correlations with silique wall thickness, silique length, silique width, beak length, and seed number per silique. However,
the correlation coefficients (CV) were rather small indicating the selection potential of varieties with high SSRI and fine silique
traits via recombination in B. napus breeding programs.
Keywords: Brassica napus L.; Silique shattering resistance; Variety screening
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 1 229 () 
Fig. 1 Distribution of shattering resistance index of 229 accessions
in B. napus
SSRI 0~0.1 ; SSRI 0.1~0.3 ;
SSRI 0.3~0.5 ;
SSRI 0.5~0.7 ; SSRI 0.7 
The accessions with SSRI between 0 and 0.1 belong to very susceptible
group; those with SSRI between 0.1 and 0.3 belong to susceptible group;
those with SSRI between 0.3 and 0.5 belong to the medium group; those
with SSRI between 0.5 and 0.7 belong to relatively resistant group; those
with SSRI over 0.7 belong to resistant group.
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Table 1 ANOVA of the silique shattering resistance index (SSRI) of 6 representative varieties (lines) based on
the tests in 2005–2007
 Source  df  SS  MS F F-value P > F
 Replicate 1 0.0000 0.0000 0.0000 0.9460
 (!) Accession 5 2.4560 0.4912 131.8800 0.0001
# Year 2 0.1343 0.0671 18.03 0.0000
 (!)× # Accession × year 10 0.3418 0.0342 9.180 0.0000
$ Error 17 0.0633 0.0037
%& Total 35 2.9954


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Table 2 Variance of 9 silique traits and correlation coefficients between shattering resistance indexes and 9 silique traits
()
Silique trait
*+,-
Variance range

Mean
./012!0
Correlation coefficient
3 Silique number per centimeter(cm-1) 0.5424−2.5573 1.5203 −0.1582 **
4.56789 Upper angle between stalk and stem() 40−150 80.43 −0.0436
4.789 Upper angle between stalk and silique() 115−180 155.02 −0.0044
4: Stalk length(cm) 0.84−3.17 2.12 0.0235
: Silique length(cm) 2.98−11.50 7.08 0.3394**
; Silique width(cm) 0.365−0.660 0.507 0.2299**
<: Peak length(cm) 0.67−2.33 1.25 0.2635**
=> Silique wall thickness(mm) 0.05−0.38 0.17 0.2708**
?0 Seeds per silique 2.38−39.00 23.75 0.1668**
**
Significant at P<0.01.

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