绿豆象是对绿豆危害最严重的仓储害虫，检测和利用抗绿豆象基因是控制该害虫最经济有效的方法。利用高感豆象绿豆栽培种Berken和高抗豆象绿豆野生种ACC41亚种内杂交得到的重组近交系（RIL）群体及据此构建的1个包含79个RFLP分子标记的遗传图谱，通过对3个种植环境条件下收获的RIL群体进行2年的室内人工接虫鉴定，评价其抗绿豆象表现。QTL作图结果表明，在第9连锁群mgM213~VrCS161标记之间的1个QTL在2年3点试验中均被检测到，贡献率在74.05%~79.27%，是抗绿豆象主效基因。该QTL的加性效应值为负，表明来自父本ACC41的这个位点的等位基因可以提高绿豆对绿豆象的抗性。本研究结果对开展绿豆抗绿豆象育种以及抗绿豆象基因的精细定位和克隆有重要意义。
Mungbean [Vigna radiate (L.) Wiclzek] is one of the main edible beans in China while bruchid are serious devastating stored pests of grain legumes causing considerable loss to mungbean. The most important of the bruchid beetle in Asian is Callosobruchus chinensis (L). Breeding for bruchid-resistant cultivars is a major goal in mungbean improvement. In this study, we analyzed the QTLs, using a Recombinant-inbred-lines (RIL) population from the cross between a highly susceptible economical parent Berken and a highly resistant wild parent ACC41. The RIL population together with their parents were planted in three sites (Australia, Beijing and Nanjing) and evaluated for their resistance by infesting with C. chinensis in 2005 and 2006 in our laboratory. We set the combination of a plant site and a year as an environment, so there are four environments (Australia 2005, Australia 2006, Beijing 2006, and Nanjing 2006). Chi-square analysis (χ2) of the RIL population showed that except for the accessions planted in Beijing field and tested for resistance in 2006, the accessions in other three environments supported the 1:1 segregation ratio. This clearly indicated there was a major resistant gene in ACC41 suitable for mapping. A genetic linkage map with 79 RFLP markers was constructed. Software WinQTLCart was applied to detect QTLs. As a result, a QTL named brI on group 9 steadily existed in the four environments and LOD was 12.99 to 115.12, explaining 74.05 to 79.27% of the phenotypic variations. It is a major QTL for bruchid resistance. The additive effect of the QTL was negative indicating that the allele of the loci from ACC41 promotes the resistance. It will be beneficial to bruchid-resistant breeding and plays an important role in mapping and cloning of the bruchid-resistant genes.
全 文 ：