利用牙签接种和花瓣接种鉴定了甘蓝型油菜DH821(抗)×DHBao604(感)组合P1、P2、F1、BC1、BC2、F2 6个世代群体菌核病的抗性,通过主基因-多基因混合遗传模型分析表现,牙签接种3 d的抗性受2对加性主基因+加性-显性多基因控制,BC1、BC2、F2 群体主基因遗传率为12.49%~28.48%,多基因遗传率为21.08%~27.87%;5 d的抗性由2对加性-显性-上位性主基因控制,显性及显性互作效应明显,无多基因修饰,BC1、BC2、F2群体主基因遗传率为25.19%~38.69%;3~5 d的菌丝扩展抗性由2对加性-显性-上位性主基因+加性-显性多基因控制,主基因效应明显,多基因效应不明显,BC1、BC2、F2 群体主基因遗传率为0.72%~54.06%,多基因遗传率为7.34%~46.79%。花瓣接种15 d的抗性由2对加性-显性-上位性主基因+加性-显性-上位性多基因控制,2对主基因的加性效应和显性效应为-3.6051~ -3.5225,互作效应为3.5190~3.6089,主基因遗传率为0.91%~41.79%,多基因遗传率为19.32%~73.69%。
Resistance to sclerotinia sclerotiorum (Lid) de Bary in cross of DH821×DHBao604 (Brassica napus L.) was identified by mycelial toothpicks and petals inoculations, and the inheritance of resistance in this cross was analyzed by applying the major gene plus polygene model of quantitative traits to a joint analysis of multi-generations (P1, P2, F1, BC1, BC2, F2). The results showed that resistance measured 3 d after mycelial toothpicks inoculation was controlled by 2 pairs of major genes with additive effects plus polygene with additive-dominant effects. Heritability values of the major genes of BC1, BC2, and F2 populations were from 12.49% to 28.48%, while these of polygene were from 21.08% to 27.87%. Resistance measured 5 d after inoculation was controlled by 2 pairs of major genes with additive-dominant-epistatic effects, among which dominant and associated epistatic effects were significant. Heritability values of the major genes of BC1, BC2, and F2 populations were from 25.19% to 38.69%. Resistance to expansion of mycelia during 3-5 d after inoculation was controlled by 2 pairs of major genes with significant additive-dominant-epistatic effects plus polygene with low additive-dominant effects. Heritabilities of major genes and polygene in BC1, BC2, and F2 populations were from 0.72% to 54.06% and from 7.34% to 46.79%, respectively. Resistance detected 15 d after infested petals inoculation was controlled by 2 pairs of major genes with additive-dominant-epistatic effects plus polygene with additive-dominant-epistatic effects. The additive and dominant effects of the major genes were from -3.605 to -3.5225, and the interaction effects from 3.5190 to 3.6089. Heritability of major genes and polygene in BC1, BC2 and F2 populations were from 0.91% to 41.79% and from 19.32% to 73.69%, respectively.
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