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作 者 ：林良斌;官春云;李栒;王国槐;周小云;陈信波

期 刊 ：作物学报 1999年 25卷 04期 页码：447-450

关键词：甘蓝型油菜；遗传转化；BT毒蛋白基因；子房注射法；

Keywords:Brassica napus, Genetic transformation, BT toxic protein gene, Ovary injection,

摘 要 ：以甘蓝型油菜湘油13为试验材料，运用子房注射法将BT毒蛋白基因导入油菜。在授粉后第20小时至第30小时用自制的微玻针从子房中部注射0.5~1.5μg外源DNA，以高达12.8%的频率获得抗卡那霉素植株。分子杂交分析证明BT毒蛋白基因已整合到油菜基因组中，转化频率为1.6%，表明子房注射法是一种有效、实用的油菜遗传转化方法。

Abstract:Using Brassica napus cv. Xiangyou 13 as materials，BT toxic protein gene was introduced into oilseed rape via ovary-injection. Anti-kanamycin plants were obtained at highest frequency of 12.8% when 0.5~1.5μg foreign DNA was injected into the middle of ovary from 20th hour to 30th hour after pollina

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