The Analysis of the Biological Characters in Hexapod hybrids Derived from Brassica carinata and B. rapa-文献传递-植物通论文库

摘要：通过杂交和染色体加倍人工合成了埃塞俄比亚芥×白菜型油菜间的三倍体和六倍体杂种，并对其进行了细胞学鉴定、气孔观察以及一些生理生化特性的分析。在染色体记数、原位杂交和分子标记印证的基础上，比较了两种不同染色体加倍方法的效率；生理生化分析显示，六倍体杂种植株叶片的游离脯氨酸含量、质膜相对透性、Vc含量和叶绿素含量与三倍体杂种植株相比都有明显的差别。

Abstract:Interspecific hybridization between Brassica species is an important way to create new resources and combine the valuable characters of different species, which is widely used in plant breeding. 120 plants of triploid hybrid (ABC, n=27) derived from cross between B. carinata (BBCC, 2n=34) and B. rapa(AA,2n=20)was obtained which played a important role in creating bridge materials for other Brassica species with one or two of the three genomes, 80% of the hybrid plants had expected 27 chromosomes and showed intermediate characteristics. RAPD markers also verified the true hybrid nature. Two waye were used for chromosome doubling, one was that the 0.05% colchicine aolution was dropped on the growing point, another was that the seedlings were dipped into the 0.05% colchicine solution for 8 hours, the ratio for chromosome doubling varied much between two methods.... ...

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栗茂腾等：埃塞俄比亚芥与白菜型油菜间六倍体杂种的获得及其生物学特性研究图版Ⅰ
LI Mao-Teng et al.: The Analysis of the Biological Characters in Hexapod hybrids Derived from Brassica
carinata and B. rapa PlateⅠ

图版Ⅰ说明
1：埃塞俄比亚芥与白菜型油菜的三倍体杂种体细胞具 27条染色体；2：埃塞俄比亚芥、白菜型油菜及其杂种的形态比较；3：埃塞俄比亚芥与白
菜型油菜杂种的加倍植株（六倍体）体细胞具 54条染色体；4：六倍体杂种体细胞的原位杂交，其中 16条染色体具有很强的杂交信号（箭头）；5，
6：杂种加倍植株中出现的非整倍体细胞，染色体数目分别为，2n=71 和 2n=51；7，8：分别表示六倍体和三倍体杂种植株的气孔大小；9：显示
个别较大的气孔（保卫细胞，箭头所示）被一群较小的气孔所包围。（图中横线代表 5 µm）；10：引物 S1076在杂种植株以及亲本 10167和 9C51
的 RAPD扩增图谱（M为分子量标记，1~22为不同杂种，23为 9C51，24为 10167）。
Explanation of PlateⅠ
Fig.1：The 27 chromosomes of hybrid (triploid) derived from Brassica carinata and B. rapa; Fig.2：The morphology of the hybrids between B. carinata × B.
rapa and the parents; Fig.3：The 54 chromosomes of hexaploid; Fig.4：The GISH plate of hexaploid in which 16 chromosomes with strong signals (Shown
by the arrow); Fig.5, 6：Aneuploids derived from doubled hybrids with chromosome number of 2n=71 and 2n=51; Fig.7, 8: The stomata of hexaploid and
triploid respectively; Fig.9: An enlarged single stomata (guard cell) was surrounded by a group of small stomata; Fig.10：RAPD profile of hybrids between
10167 and 9C51 with the primer of S1076 (M: molecular marker; 1－22: different hybrid individuals; 23: 9C51; 24:10167). (The horizontal line in the plate
represents 5 µm).
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The Analysis of the Biological Characters in Hexapod hybrids Derived from Brassica carinata and B. rapa

埃塞俄比亚芥与白菜型油菜间六倍体杂种的获得及其生物学特性研究

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