Abstract:In cell suspension culture of Panax ginseng C.A.Mey, when non-ionic water was used instead of double distilled water, cell growth rate and oligosaccharin yield were decreased about 2.3% and 2.9% against controlled, respectively, when defined sugar was used instead of sucrose, cell growth rate and oliogsaccharin yield were decreased about 1.74% and l.23% against controlled, respectively. As a result, substitutive medium involving non ionic water and defined sugar was used instead of original medium in involving redistilled water and sucrose. When cells were suspended in the substitutive, cell growth rate and oligosaccharin yield were 0.509 gDW / L.d and 1,443g / L, respectively, which were slightly decreased by 1.74% and 1.23% as compared with control in cell suspension culture, while the cost of medium could be largely reduced about 3000 yuan/ t medium. Time course of cell suspension culture in substitutive medium was been unchanged, an appropriate period for oligosaccharin preparation from P.ginseng cultured cells was about 20 day.