Abstract:From the tubers of Gastrodia elata Bl.,collected in Guizhou Province,four compounds together with β-sitosterol and dauocsterol were isolated.Their structures were determined as follows as based on spectral data:gastrodamine[di-(p-hydroxyl benzyl)hydroxylamine,](I),L-pyroglutamic acid (II),6-methyl citrate(III) and 1,5-dimethyl cirate(IV). Among them gastrodamine (I) us a new compound,compound II and IV are isolated from this plant for the first time.