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作 者 ：浦帆；江明；张正居；张劲松；孔繁浩

期 刊 ：云南植物研究 1998年 20卷 03期 页码：1-3

关键词：香荚兰；糖甙分解；HPLC分析；香兰素；酶促生香；

Keywords:Vanilla planifolia,

摘 要 ：采用HPLC分析监测不同生香阶段和陈化期的香荚兰（Vanilla planifolia Andrews）豆荚中香兰素等4个糖甙水解成分的含量变化。用β-葡糖甙酶处理香荚兰青美，处理后之豆荚中，香兰素等4个糖甙水解成分的含量均远高于对照。对加酶量、酶促反应时间、反应温度、pH值4个关键因素进行条件筛选研究，并在所选最佳反应条件下，对不同生香阶段的豆荚进行酶处理。结果表明，酶促生香效果明显，可促进香兰素糖甙等物质的分解，提高豆荚中香兰素含量，缩短生香陈化时间，具有潜在的生产应用价值。

Abstract:The content and its variation of 4 components from glucosides hydrolysis such as vanillin in the Vanilla bean of different curing stages have been analyzed by HPLC. The components in green bean are in higer content upon (3- glucosidase treatment. Four important factors for the enzymatic hydrolysis were selected by tests. The bean in different curing stages was treated by the enzyme under selected condition. The result shows that it can improve the hydrolysis of glucosides and increase vanillin content in Vanilla bean.

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