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作 者 ：黄遵锡；慕跃林；周玉敏；陈文久；许坤一；余尊祥；卞云萍；杨桂仙

期 刊 ：云南植物研究 1997年 19卷 03期 页码：1-3

关键词：短叶红豆杉；紫杉醇；发根农杆菌；毛状根培养；

Keywords:Taxus brevifolia,

摘 要 ：发根农杆菌（Agrobacterium rhizogenes）A4（ATCC 31789)感染短叶红豆杉（Taxus brevifolia）芽外植体，30～35 d后可诱导出毛状根，40 d后转化率可达30％，毛状根经农杆碱单克隆抗体酶联免疫分析，证明已被转化。毛状根生长速度较快，5株毛状根在无激素的B5液体培养基中悬浮培养 20 d，生物量平均增加约 9倍，是同等条件下短叶红豆杉愈伤组织液体悬浮培养的2.9倍。经紫杉醇单克隆抗体酶联免疫反应检测，毛状根中含有紫杉醇，其含量为短叶红豆杉愈伤组织的1.3—8.0倍，悬浮培养过程中，毛状根也向培养液中分泌 0.01～0.03 mg／L的紫杉醇。

Abstract:This paper reports the establishment of an efficient transformation system for Tax us brevi,folia, using Agrobacterium rhizogenes A4(ATCC31798). Hairy roots are induced from shoot explants after 30- 35 d inoculation with the bacteria. Frequency of the explants transformation by A4 is 30% after 40 d. Transformation is confirmed by agropine monoclonal antibody ELISA ana-lysis. The average value of biomass of hairy root cultures suspended in hormone free B5 medium increases 8.67 times after 20 d culture. It is 2.9 times as much as callus cu1ture from T.brevifhlia in the same culture condition. The hairy root culture can synthesize taxol whose content is l.3- 8 times as much as that in the callus from T. brevifolia. Taxol can also be detected in growth me-dium.

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