Abstract:The karyotype and differentiation of the four local populations in Lilium duchartrei Franch. from Hengduan Mountain area of northwestern part of Yunnan Province were analysed in this study. No chromosomal number variaton was observed and all karyotype asymmetries be-long to 3B type. Karyotype polytypism among the four populations was displayed as the chromosomal heterozygosity, and the number variation as well as position shift of the secondary constrictions and the satelites. As a result, there is high intraspecific differentiation in Lilium duchartrei and we asume that Hengduan Mountain area is its origin and differentiation center.