Abstract:On the studies of the 1H NMR spectra of steroidal sapogenins which have some hydroxy groups in ring A and B, we found that 10--OH tsolvent shift of d5-pyridine on 19-Me are about 0.30 ppm, in △5-series; 1β, 5β, and 6β OH solvent shifts of d5-pyridine on 19-Me are about 0.15 ppm, respectiyely, in 5β-series. Comparing with the sapogenins which have no OH of 1β-, 5β-, and 6β-positions, we found that the 19-Me chemical shifts (in C,D,N) of sapogenin- shaving 16-OH, 1β-. 5β-OH, and 1β-, 5β-, 6β-OH shift to lower field about 0.3, 0.6 and 0.9 ppm, respectively.