Abstract:In this paper, a total of 24 SSR markers distributed on 12 chromosomes were used to construct Ningxia rice microsatellite markers database with 47 varieties which identified carefully. 144 alleles were revealed，and 3 to 11 alleles were identified among different loci．The mean number of alleles per locus was 5.14. The average Nie genetic diversity index was 0.6187. The 28 loci were differences between the tested material. There is only one locus has difference between Ningjing 28 and Ningjing 23, Ningjing 23 and Ningjing 35. Two or more loci have differences between remaining varieties, accounting for 93.6% of the tested material. 10 pairs of core primers were selected from 28 pairs of primers which at least one pair of primers has difference between varieties, and varieties can be distinguished one by one. Using 10 core markers, in the same position on the migration to 1 and 0 mark represent yes and no, Ningxia rice DNA fingerprinting database was established.