---

作 者 ：胡忠,黄仕周,庄承纪

期 刊 ：云南植物研究 1980年 2卷 02期 页码：1-3

关键词：花粉；白苗；化学；诱变剂；

Keywords:causes, pollen, culture, chemical, plantets,

摘 要 ：用化学诱变剂甲基磺酸乙酯（EMS）、乙烯亚胺（EI)和1-甲基-3-硝基-1-亚硝基胍(MNNG)处理培养早期的水稻花药，观察到花粉白苗的形成增加而绿苗的形成减少。因此花粉总苗数中白苗所占的百分数显著增高，例如用EMS 2.5 ml／1在26℃处理品种8126的培养花药，苗中白苗为34.9％，而对照为6.7％。但即使在这种情况下，所培养出的花粉植株中只有很少数发生性状突变。白苗也在少数绿色花粉植株后代中出现，其频率第二代比第三代高100倍以上。试验结果有利于认为水稻白化苗的成因可能是培养花粉中某些基因的缺失。化学诱变剂处理导致较多花粉发育成白苗，这对于试图利用水稻培养花粉进行诱变以便获得某种突变体是一大障碍．

Abstract:Treating the cultured anthers in early stage with mutagens ethyl methane sulphonate(EMS), 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) and ethylene imide (EI), we observed an enhance in the formation of albino pollen plantlets with an decrease in the formation of green plantlets, and thus an obvious increase of the percentage of albinos in total plantlets, for instance, 34.9% in treatment of 2.5 ml/1 for 8 h. (26℃) on cultured anthers of variety 8126,compared with 6.7% of control. But even in this case, mutations in other characters was observed only in very few pollen plants. Albino seedlings also occurred in progenes of a few pollen plants, the frequency of its occurence in H2 strains was 100 times more than that in H3. The experimental results are available to the claim that the cause of albinos formation might be the losses of somc genes. The formation of more albino pollen plantlcts callqed by mutagens would give much difficulty to the attempt of producing mutants through in-ductive mutation on cultured pollens of rice.

---