摘 要 ：LBD是一类具有LOB（lateral organ boundaries）结构域的基因家族，在植物发育过程中起到非常重要的作用。采用生物信息学方法，根据拟南芥LBD基因序列鉴定了普通烟草基因组中的LBD基因，并对家族成员进行了序列特征、系统发育和表达谱分析。结果表明：普通烟草基因组中共有98个LBD基因成员，其基因结构相对简单，一般含有1-3个外显子。LBD基因家族可分成I和II两大类，两类均含有CX2CX6CX3C保守结构域，但II类不含有LX6LX3LX6L形成的“卷曲螺旋”二级结构，根据与拟南芥LBD蛋白构建的系统发育树则可细分成5个亚家族（Ia、Ib、Ic、Id和II）。将普通烟草LBD基因与表达序列标签（EST）比对，发现36个基因有EST证据；EST、芯片数据和转录组数据分析表明：LBD基因具有不同的组织表达模式，部分基因表现出组织特异性。这些研究结果为普通烟草LBD基因家族功能的深入研究奠定了基础。
Abstract:LBD gene with LOB (lateral organ boundaries) domain structure, which plays very important role in plant development. Base on bioinformatic method, the LBD genes of Nicotiana Tabacum were screened with the LBD gene sequences of Arabidopsis. The sequence structure, phylogenetic relationship and expression pattern of the identified gene sequences were analyzed. The result show: a total of 98 LBD genes were identified from tobacco with simple genetic structures contained 1-3 exons generally. Tobacco LBD gene family be divided into two types (I and II). All LBD members had conserved domain CX2CX6CX3C, however, the type II genes had secondary structure of coiled coil made of LX6LX3LX6L. Furthermore, LBD genes can be divided into 5 sub families (Ia, Ib, Ic, Id and II) by their phylogenetic relationship with Arabidopsis. 36 members have EST evidenc; the analysis of EST, array data and transcriptome show that the LBD genes had different expression patterns and tissue specificity. These results provide abundant information for the LBD genes family further functional analysis.