摘 要 ：摘要：植物多药和有毒化合物排出家族（multidrug and toxic compound extrusion，MATE）是一类可转运毒素、金属离子、次级代谢产物的次级转运蛋白家族。该家族主要在植物的解毒机制中发挥作用，部分成员也参与植物的形态建成过程。MATE家族在烟草基因组中的数量、特征及功能目前尚未开展系统分析。本研究利用生物信息学方法对普通烟草（Nicotiana tabacum）基因组中的MATE基因进行了预测分析，共预测到131个基因，分为4个亚家族。亚家族3在进化树中形成较为独立的分枝，其跨膜区数量、亚细胞定位、保守结构域方面与其他亚家族不同。转录组数据显示，相当一部分MATE家族基因在所有组织中低量表达。GO功能注释结果表明该家族成员主要作为一种转运体，在应激响应、生物调控等过程中发挥作用。本研究为烟草及其他植物中MATE家族的鉴定和功能研究提供了数据基础。
Abstract:Abstract: Multidrug and toxic compound extrusion (MATE) proteins, comprising the most recently designated family of multidrug transporter proteins, are identified, characterized and found to be involved in the detoxification of endogenous secondary metabolites and xenobiotics. Recent researches also showed that some of the MATE members play essential role in plant development and organ initiation. So far, the MATE family in common tobacco (Nicotiana tabacum) hasn’t been further studied. In this research, 131 MATE genes were preliminary identified in common tobacco by bioinformatics, and they were classified into 4 subfamilies. We also found that subfamily 3 was distinct from other subfamilies, including the conserved domain prediction, TM prediction and subcellular location prediction, there was the same situation in subfamily 3 in Arabidopsis. Many MATE genes showed low expression level in all tissues by transcript analysis. GO analysis indicated that as a kind of transporters, the MATE family members are involved in a series of biological processes such as stimulus responses and biological regulation. The results we got will be helpful for the further analysis of the MATE family proteins in other plants.