---

期 刊 ：植物遗传资源学报 2015年 16卷 2期

关键词：大白菜；EMS；突变体库；诱变育种；

Keywords:Chinese cabbage, EMS, mutant library, mutantion breeding,

摘 要 ：大白菜突变体的获得是种质创新的有效途径，也是开展功能基因组研究的良好基础。本研究选取自交亲和、易于进行小孢子培养的高代自交系‘A03’作为大白菜突变体库构建的野生基因型材料。针对EMS种子处理对M1和M2代种子和幼苗活力的影响进行了分析，并进一步针对突变体库M2群体结球期、收获期和生殖生长阶段表型性状变异进行了研究。结果表明，以浓度0.4%的EMS浸泡处理种子16 h，以及连续对两代种子进行诱变处理，均可以作为构建大白菜突变体库的适宜诱变方案获得的大白菜突变体库M2群体植株的结球期、收获期和生殖生长阶段表型性状变异丰富，变异频率分别为21.65%，22.40%和25.65%。

Abstract:Creating mutants is an effective way of germplasm innovation and useful basis of functional genomics research in Chinese Cabbage. In this study, the inbred line ‘A03’ of Chinese cabbage was selected as wild genotype for constructing one mutant library because of its self-compatibility and easy microspore culture. The influences of EMS treatments on vigor of M1 and M2 seeds and seedlings were investigated. The variations in phenotypic traits of M2 population at heading stage, harvest stage and reproductive growth stage were further investigated. The results showed that the more suitable mutant scheme of creating mutants was considered to soaking seeds with EMS 0.4% and with time 16 h. The way of continuous treatments with EMS on seeds of two generations could be used in constructing mutant library of Chinese cabbage. Within the obtained mutant population, there was wide variation in phenotypic traits of M2 individuals at heading stage, harvest stage and reproductive growth stage, with mutation frequencies of 21.65%, 22.40% and 25.65%, respectively.

---