---

期 刊 ：植物遗传资源学报 2014年 15卷 3期

关键词：萝卜；分子身份证；SSR；

Keywords:Radish, molecular identification, SSR,

摘 要 ：从基于萝卜EST开发的SSR引物中，筛选出22对SSR引物对75份来源和特征不同的代表性萝卜种质进行鉴定，共扩增出条带153条，其中87条带为多态性，多态性位点百分率为55.49%，平均每对引物可扩增出6.95条带和3.95条多态性带。有效地显示出每份萝卜种质的特异性。基于最少引物鉴定最多种质的原则，利用MATLAB程序筛选出8对SSR引物，依据8对引物的扩增数据，经过多态性谱带的有序编码转换，构建出75份萝卜种质分子身份证。显示利用SSR标记构建萝卜种质分子身份证进行种质资源的鉴定和保护是可行的。

Abstract:153 bands were generated among 75 radish germplasms using 22 pairs of SSR primers which were screened out. Of them, 87 bands were polymorphic. The percentage of polymorphic alleles was 55.49%. On average, the number of alleles per SSR locus was 6.95 in total and 3.95 of polymorphic bands The genetic diversity among these radish germplasms was rich. After raw data processing and filtering by MATLAB, 75 radish germplasm could been distinguished and their molecular identities were constructed usingr 8 pairs of SSR primers. It showed that it is feasible to employ SSR markers to construct the molecular identities for elite germplasm resource and new varieties in radish.

---