Abstract:Two new iridoid glycosides, scrospioside A and B along with known harpagoside and p-methoxycinnamic acid were isolated from the roots of Scrophularia spicata Fr., a Zang (Tibetan) nationality‘s folk medicinal plant in Yunnan. By the spectral data, their structures were elucidated as 6-0-α-L-[2,4-diacetyl-3-trans-cinnamoyl]-rhamnopyranosyl catalpol and 6-0-α-L-[2-acetyl-3-trans-cinnamoyl]-rhamnopyranosyl catalpol, respectively.