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期 刊 ：植物遗传资源学报 2013年 15卷 6期

关键词：长尖叶蔷薇；居群；遗传多样性；SSR；

Keywords:Rosa longicuspis Bertoloni, Population, Genetic diversity, SSR,

摘 要 ：采用SSR标记对云南地区的8个长尖叶蔷薇天然居群进行了遗传多样性分析。结果显示：所选用的14对SSR引物，共检测到77个等位位点；在物种水平上，总居群的Nei’s基因多样性指数（He）和香农指数（I）分别为0.3139和0.4747；该居群内遗传变异（65.47%）大于居群间遗传变异（34.53%），说明居群内变异是其居群的主要变异来源；利用Popgene计算出两两居群间的Nei’s遗传一致度（I）和遗传距离(D)，其范围分别为0.7879~0.8986和0.1070~0.2384，依据遗传距离可将8个居群分为3组，8个居群并没有严格依据地理距离的远近而聚类；海拔与Nei’s基因多样性的相关系数为0.8771（P＜0.01），呈显著正相关。研究结果表明，云南地区的长尖叶蔷薇居群遗传多样性较高，居群间遗传变异存在中度的遗传分化。基于得到的居群遗传信息，建议采取就地保护为主的保护策略，但当个别居群野外的生存环境被自然或者人为因素破坏时，建议采取迁地保护的保护策略。

Abstract:We analyzed the genetic diversity of eight natural populations of Rosa longicuspis Bertoloni in Yunnan by simple sequence repeats (SSR) markers. The result showed that: 77 alleles were detected by 14 polymorphic SSR primers. At the species level, the Nei‘s genetic diversity (He) was 0.3139 and the Shannon information index (I) was 0.4747 abundant. It was 65.47% of genetic variation within populations, and 34.53% of genetic variation among populations. So the genetic variation within populations was the main cause of the genetic variation. The Nei’s pairwise genetic identity were 0.7879-0.8986, and genetic distance 0.1070-0.2384. The eight populations were clustered into 3 groups based on genetic distance, showing that the genetic distance was not significantly affected by the geographic distance. The elevation of population growth was positively correlated with He, with the correlation coefficient 0.8771(P＜0.01). The results reveal that the genetic diversity of the populations of Rosa longicuspis Bertoloni in Yunnan is abundant and the genetic variation among populations has medium genetic differentiation. Conservation strategy should be included in situ conservation and ex situ conservation based on the observed genetic information of populations.

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