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期 刊 ：植物遗传资源学报 2016年 17卷 3期

关键词：谷子；ARF；生物信息学分析；蛋白结构；

Keywords:setaria italica, ARF, information analysis, protein structure,

摘 要 ：生长素应答因子（auxin response factors，ARF）是一类可以结合在生长素应答基因启动子区域的转录因子，在植物的生长发育中起至关重要作用。本研究以谷子为材料，从谷子中鉴定出24个ARF基因并命名为SiARFs。利用生物信息学对谷子SiARFs基因的结构、染色体分布、基因倍增模式、系统进化以及基因的表达模式进行分析。结果表明，SiARF基因家族在染色体上不均匀的分布，在除2号染色体外的其它染色体上都有该家族基因，基因的扩增模式为分散复制与片段复制。SiARFs基因家族具有相对保守的结构，即包含一个保守的B3 DNA结构域，ARF结构域和Aux/IAA结构域，ARF蛋白的3D结构含有3个α螺旋和7个β折叠结构。进化树分析表明谷子ARF蛋白和物种相近的高粱、玉米聚在一起。大多数ARF基因在根、茎、叶和穗中都有表达，可能和基因参与植物的多方面代谢过程密切相关。

Abstract:Auxin response factors (ARF) are important transcription factors that can regulate the expression of auxin genes and influence the plant growth. ARFs regulate the transcription of auxin-responsive genes through binding to their promoters. In this study, a total of 24 ARF genes are identified and analyzed and named SiARF. A comprehensive overview of the SiARFs is undertaken, including phylogenetic analysis, the gene structures, chromosome locations, conserved motifs of proteins and gene expression.Twenty-four setaria italica ARF genes are distributed in all setaria italica chromosomes except chromosome. Setaria italica ARF proteins share one B3 DNA binding domain, which consists of threeαhelixes and sevenβsheets. And some of these proteins have a Aux/IAA domain and ARF domain. In addition, setaria italica ARF proteins are compared with the proteins in other species in order to discover the evolution of ARF genes. Our results also show that the expression of SiARF genes is diverse in different tissues.

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