摘 要 ：本研究采用ISSR分子标记的方法，对‘达维’、‘辽榛3号’等17个平欧杂种榛主栽品种（系）进行了遗传关系研究。实验从60条ISSR引物中筛选出7条进行电泳分析，使用NTSYS pc 2.11F和Popgene 1.32软件进行数据统计分析并作图。结果显示：7条ISSR引物共获得58条谱带，平均每条引物8.3条谱带，引物平均多态带比率84.6%；7条ISSR引物可将所有样品完全分开，当阈值为0.687时可将样品分成3组，第一组14个品种（系）可分成4个亚组，第二组1个品种，第三组2个品系；样品间的遗传相似性系数为0.448-0.879（平均0.678），群体的有效等位基因数、基因多样度、Shannon信息指数分别为1.6751、0.3701和0.5308。上述结果表明，平欧杂种榛是一个具有高度遗传多样性的群体，主栽品种（系）间存在复杂的亲缘关系。该研究结果可为其他榛属植物的相关研究提供参考。
Abstract:The objective of this study is to establish ISSR analysis system for Ping’ou hybrid hazelnut and analyze the genetic relationship for the main cultivars/varieties. 17 main cultivars/varieties, such as ‘Dawei’ and ‘Liaozhen No. 3’, are selected as materials. 7 ISSR primers are selected from 60 primers with high polymorphism, strong signals and clear bands. NTSYS pc 2.11F and Popgene 1.32 software are used to conduct the data analysis and plotting. The results show that: Totally 58 bands are obtained. The average number of bands is 8.3 per primer and the average percentage of polymorphic bands is 84.6%. All the samples are divided into 3 groups when the threshold value is 0.687. The first group, containing 14 cultivars/varieties, can be divided into four sub-groups, the second group contains 1 cultivar and the third group contains 2 varieties. The similarity coefficient of the samples varies from 0.448 to 0.879, with an average is 0.678. The effective number of alleles, Nei‘s gene diversity and Shannon‘s information index of the main cultivars/varieties are 1.6751、0.3701 and 0.5308, respectively. Ping’ou hybrid hazelnut is a high polymorphic population and the genetic relationships of the main cultivars are complex. The result of this study can offer reference for the relative researches in Corylus.