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作 者 ：罗建平 郑光植

期 刊 ：云南植物研究 1995年 17卷 01期 页码：1-3

关键词：人参；寡糖素；变异克隆系；过氧化物酶同工酶；

Keywords:Panax ginseng,

摘 要 ：用2mmol/L的MNNG处理经过滤的人参悬浮培养细胞1小时后，细胞存活率下降显著，细胞克隆植板率只是对照组的10.12%。经细胞平板克隆共获得克隆系151株，其中很多克隆系在转移培养中生长缓慢，甚至不生长而死亡。经分析可供测定的克隆系生长和寡糖素含量的差异，对11株寡糖素含量较高克隆系经连续10代继代培养观察，选出一株稳定高产人参寡糖素优良克隆系PGMB－37，其平均生长速率是0.558gDWL-1d-1，为亲本的1.5倍，平均寡糖素含量是14.67%DW，平均寡糖素产率是 2.456g/L，分别比亲本高70%和156%，并且它的过氧化物酶同工酶谱特征与亲本之间也呈稳定性差异。

Abstract:The suspension cells of Panax ginseng which had been filtrated, were treated with mutagenic compound, N-methyl-N‘-nitro-N-nitrosoguanidine at 2 mmol / L, for 1 hour. Cell viability was remarkablely decreased and the formation of cell clone was only 10.12 of control. Cells treated were plated on a nutrient agar medium. More than 150 clone lines were obtained af-ter plating culture of 60 days, of which many clone lines grew slow or stopped growing and died during transplantation culture.

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