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期 刊 ：植物遗传资源学报 2015年 16卷 4期

关键词：白檀；ISSR；自然居群；遗传多样性；

Keywords:Symplocos Paniculata, ISSR, natural populations, genetic diversity,

摘 要 ：采用ISSR分子标记记技术分析了湖南省5 个地区6 个居群白檀的遗传多样性水平和遗传结构。 结果表明，9条ISSR引物对所有居群的149份白檀样品PCR扩增检测到122个扩增位点，其中多态位点113个，多态性比率高达92.62%。Nei’s基因多样性指数（He）为0.3264，Shannon多样性指数（I）为0.4873，居群之间产生较大的遗传变异（Gst=0.5215，Nm=0.4588），AMOVA分子差异分析表明白檀居群间遗传分化程度高,51.07%的变异存在于居群间,49.83%的变异存在于居群内；UPGMA聚类分析将6个白檀居群分为3大类：大围山和龙山居群为一类，岳阳和道县居群组成另一类；衡山居群单独成一类，聚类原则跟地理位置不一致，与海拔有一定的相关性。

Abstract:The genetic diversity and structure of 6 natural populations in Hunan Province from 5 different regions was investigated using inter-simple sequence repeat(ISSR)markers.As a result,122 loci were identified from the 9 selected primers in 149 individuals of all populations,of which, 113 loci were polymorphic,The percentage of polymorphic bands (P) was high to 92.62%,while Nei’s gene diversity(H) and Shannon information index(I)were 0.3264 and 0.4873. The coefficient of gene differentiation (Gst)was0.5215,Gene flow among populations(Nm)was 0.4588, Analysis of molecular variance(AMOVA)demonstrated that the genetic differentiation among populations was relatively high. The among-population component accounts for 51.07%of the total variation, while the within-population component accounts for 49.83%. 6 populations were clustered into 3 groups by the UPGMA cluster analysis,the result showed that Da Wei Shan and Long Shan populations were in the first group,Yue Yang and DaoXian populations were in another group,and Heng Shan population separated from all the populations in the dendrogram, the geographical distribution was not related to the cluster results, but was mutually related to the geographical Elevation.

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