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期 刊 ：植物遗传资源学报 2014年 15卷 5期

关键词：籼粳交；新种质；康丰A；稻瘟病抗性；遗传；

Keywords:Indica-Japonica crosses, new germplasm, Kangfeng A, blast resistance, inheritance,

摘 要 ：摘要：康丰A是利用带有广亲和基因的籼粳亚种间杂交育成恢复系97gk419与恢复系明恢70杂交（恢复系×恢复系），所形成的胞质正常、雄性可育，而无恢复性的特殊变异新种质，再与野败胞质不育系连续回交转育成的具有特殊核遗传背景的新型水稻三系不育系。本研究通过接种不同地区的53个稻瘟病菌系，发现康丰A对华南稻区尤其是福建稻区的稻瘟病菌系具有广谱抗性。以康丰A（同型保持系康丰B）与普感品种丽江新团黑谷（LTH）杂交，获得杂交F1和F2，分别接种稻瘟病菌系81278、Guy11、FJ2009-66和98013A。接种鉴定和遗传分析表明，康丰A对4个菌系的抗性均由一对显性抗病基因控制。等位性测定表明，康丰A抗菌系81278的基因与已知抗病基因Pi-1、Pi-2、Pi-ta和Pi-3呈非等位关系，与Pi-ta（Pi-?）呈现连锁遗传，暂命名为Pi-kf1(t)。

Abstract:Abstract: Kangfeng A is a new CMS Line with special nuclear genetic background, which was from a new variant germplasm successive backcross with a CMS-wild abortive (WA).The new variant germplasm, with normal cytoplasm, male fertile and no restoring ability, which was from a cross between an indica-japonica restorer line 97gk419 with wide compatible gene and an indica restorer line Minghui 70(Restorer line×Restorer line).In this study, the evaluation of Kangfeng A for its resistance against 53 Magnaporthe grisea strains collected from different rice cultivated regions showed that Kangfeng A had the broad-spectrum performance for blast resistance in South China rice regions, especially in Fujian. The genetic analysis of Kangfeng A showed that the blast resistance was controlled by one dominant gene, which was evaluated by inoculating F1and F2 populations derived from a cross of Kangfeng A(B)×LTH with four Magnaporthe grisea strains, 81278,Guy11,FJ2009-66 and 98013A. The allelic test indicated that the resistance gene in Kangfeng A to strain 81278 was non-allelic to known loci (Pi-1,Pi-2,Pi-ta and Pi-3 ), and was genetic linkage to Pi-ta（Pi-?）, then tentatively designated Pi-kf1(t).

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