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期 刊 ：植物遗传资源学报 2014年 15卷 4期

关键词：甘蔗；优良品种材料；高粱花叶病毒；抗病性鉴定；

Keywords:sugarcane, elite varieties/materials, Sorghum mosaic virus, resistance identification,

摘 要 ：本研究采用生长期人工切茎接种和RT-PCR检测相结合方法，于2011年和2012年2次对国家甘蔗体系近年选育的49份优良新品种（系）和19份云蔗系列优良中间材料进行由高粱花叶病毒（SrMV-HH，GenBank登录号DQ530434）引起的甘蔗花叶病的抗性鉴定与评价。结果表明，49份优良新品种（系）中，1级高抗到3级中抗的有29份，占59.18%。其中粤甘40号、粤甘42号、粤糖55号、云蔗03-194、云蔗99-596、云瑞06-189、桂糖30号、德蔗03-83、闽糖 01-77等9份材料表现1级高抗，占18.37%；福农0335、柳城05-129、粤糖96-86、云蔗01-1413、云蔗03-258、云蔗06-80、桂糖02-351等7份材料表现2级抗病，占14.29%。19份云蔗系列优良中间材料中，1级高抗到3级中抗的有13份，占68.42%。其中云蔗04-622、云蔗05-197、云蔗06-267、云蔗05-194、云蔗06-160、云蔗07-2384、云瑞05-704等7份材料表现1级高抗，占36.84%；云蔗06-362表现2级抗病，占5.26%。研究结果为深入开展甘蔗抗花叶病育种，选育和推广优良抗病品种有效防控甘蔗花叶病提供了科学依据和优良抗源材料。

Abstract:In 2011 and 2012, the resistance to sugarcane mosaic disease caused by Sorghum mosaic virus (SrMV-HH, GenBank accession number DQ530434) was identified and evaluated in 49 new improved varieties and 19 elite intermediate materials of Yunzhe series by dropping inoculation on stem-section and SrMV RT-PCR detection. The results showed that of the 49 new improved varieties, 29 (59.18%) were highly (Grade 1) to moderately resistant (Grade 3) to SrMV. Nine varieties were highly resistant (Grade 1) to SrMV, which included Yuegan 40, Yuegan 42, Yuetang 55, Yunzhe 03-194, Yunzhe 99-596, Yunrui 06-189, Guitang 30, Dezhe 03-83 and Mintang 01-77 and presented 18.37% of the 49 new improved varieties. Resistance (Grade 2) was found in 14.29% (7 out of 49) of the 49 new improved varieties, which included Funong 0335, Liucheng 05-129, Yuetang 96-86, Yunzhe 01-1413, Yunzhe 03-258, Yunzhe 06-80 and Guitang 02-351. Of the 19 fine intermediate materials of Yunzhe series, 13 (68.42%) were highly (Grade 1) to moderately resistant (Grade 3) to SrMV. In 19 elite intermediate materials of Yunzhe series, seven materials (Yunzhe 04-622, Yunzhe 05-197, Yunzhe 06-267, Yunzhe 05-194, Yunzhe 06-160, Yunzhe 07-2384 and Yunrui 05-704) were scored as highly resistant (Grade 1), counting for 36.84 % (7 out of 19). One material (5.26%), Yunzhe 06-362 were resistant (Grade 2) to SrMV. The results in the present study could provide scientific basis and valuable resistance resource for the furher breading of sugarcane cultivars against mosaic disease, and effective control of sugarcane mosaic disease by selecting and extending elite resistant cultivars.

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