Abstract:This experiment analyzed the genetic diversity of wild walnut in xinjiang by using SRAP molecular markers.Through the screening of 15 polymorphic SRAP primer combinations for PCR amplification,get the xinjiang wild walnut genetic differentiation corfficient(Gst) is 0.1152, this suggested that the most part to show that the genetic variation of wild walnut exist within the region and accounts for 88.48% of total variance; percentage of polymorphic loci was 94.07%, shannon’s information index I=0.4954,average allele Na=1.9454,showed that xinjiang wild walnut with high genetic diversity; genetic similarity coefficient among various regions between 0.8981 to 0.9496,genetic distance between 0.0553 to 0.1075,which show that there are abundant rich genetic variation between wild walnut resources. Cluster analysis results can be grouped into two categories,these fuether clarify the genetic relationship between the various.