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Evaluation for extraction process of Shenqi Tongmai Yizhi particles based on antioxidant capacity in vitro and its spectrum-effect relation

基于体外抗氧化活性的参芪通脉益智方提取工艺评价及其谱效关系的初步研究



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EvaluationforextractionprocessofShenqiTongmaiYizhiparticles
basedonantioxidantcapacityinvitroanditsspectrumefectrelation
ZHANGXiaoli1,LIUYuling1,FANLijiao1,WANGYueliang1,2,CHENKai1,2,LIHui1
(1InstituteofChineseMateriaMedica,ChinaAcademyofChineseMedicalSciences,Beijing100700,China;
2SchoolofChineseMateriaMedica,JilinAgriculturalUniversity,Changchun130118,China)
[Abstract] BasedonDPPHmethod,theantioxidantactivitiesofShenqiTongmaiYizhiparticleswithdiferentextractionprocesses
werecomparedThecontributiontotheantioxidantcapacityinvitrowasexploredbymeansofgreyrelationalanalysisondiferent
chemicalcompositionsinthefingerprintTheresultsshowedthattheIC50concentrationvaluesofwaterextract,waterextractfromalco
holprecipitation,alcoholextract,andalcoholandwaterextractwere08014,08591,07961,09180g·L-1;andthealcohol
extractisthebestmethodtoextractantioxidativecomponents,withthehighestantioxidantactivityandlowestIC50Whenthemass
concentrationoftheherbsreachedacertaindegree,itsfreeradicalclearanceratewassimilartothatofvitaminCcontrolgroupThe
orderofdiferentchemicalcontributionsofconstituentstotheantioxidantactivityinthefingerprintwas4>3>33>53>9>10>11>
34>15>59>8>61>52>20>42>18>29Thepreliminaryexplorationforthespectrumeficiencyrelationsprovidesreferencefor
studyingtraditionalChinesemedicinecompoundprocessingmethodandthepharmacodyamicmaterialbasis
[Keywords] antioxidant;DPPH;fingerprint;greyrelationalanalysis;spectrumeficiencyrelations
doi:104268/cjcmm20160911
  
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