作 者 :程潜峰,钱汉清,张定虎,黄莹,沙慧子,刘宝瑞
期 刊 :中国中药杂志 2016年 41卷 11期 页码:2093.
关键词:姜黄素 ;红细胞 ;红细胞膜 ;纳米粒子 ;抗肿瘤 ;
Keywords:curumin , erythrocytes , erythrocyte membrane , nanoparticles , anti-tumor ,
摘 要 :该文采用超声法制备一种新型可降解、生物相容性良好的负载姜黄素(curumin,Cur)红细胞膜纳米粒子(Cur-RBC-NPs)。通过比较不同参数制备所得纳米粒子的粒径来优化Cur-RBC-NPs的制备条件,并考察其形态、粒径分布、稳定性、体外释放特性、细胞对纳米粒子的摄取及体外抗肿瘤效果等。结果表明,所制得姜黄素纳米粒子为较规则的圆球形,平均粒径为(245.7±1.3) nm,包封率为50.65%±1.36%,载药量为6.27%±0.29%,具有良好的缓释特性。体外实验中,纳米粒子能够被肿瘤细胞迅速摄取,Cur-RBC-NPs组有显著的抑制肿瘤生长效果。负载姜黄素的红细胞膜纳米粒子制备方法简便,具有良好的药物缓释性和抗肿瘤能力,有望成为新型的有临床应用前景的纳米给药系统。
Abstract:The objective of this study is to develop a new-type biodegradable, biocompatible curcumin-loaded nanoerythrosomes (Cur-RBC-NPs) by means of the sonication method. The size of Cur-RBC-NPs was optimized by varying drug loading parameters. The morphology, size distribution, stability, in vitro release pattern, cellular uptake of nanoparticles and in vitro anti-tumor effects were evaluated, respectively. The results showed the prepared Cur-RBC-NPs were nearly uniform spheres, with an average diameter of (245.7±1.3) nm. Encapsulation efficiency (EE) and load efficiency (LE) of Cur-RBC-NPs were 50.65%±1.36% and 6.27%±0.29%. And the nanoparticles had a good sustained release property. According to the in vitro experiment, Cur-RBC-NPs were effectively taken in by tumor cells, and exhibited a significant anti-tumor effect. In conclusion, the method for preparing Cur-RBC-NPs is convenient, with a good sustained release behavior and anti-tumor efficacy, and so expected to be a new-type nano-drug delivery system in clinical practice.
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