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Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings

铁皮石斛内生真菌对宿主组培苗生长与代谢成分的影响



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EfectsofendophyticfungifromDendrobiumofficinaleonhostgrowthand
componentsmetabolismoftissuecultureseedlings
ZHUBo1,2,3,LIUJingjing2,SIJinping2,QINLuping3,HANTing3,ZHAOLi4,WULingshang2
(1.ZhejiangChineseMedicalUniversity,SchoolofPharmacy,Hangzhou310053,China;
2EngineeringandTechnicalResearchCenterofDendrobiumoficinaleofStateForestryAdministration,
ZhejiangAgriculturalandForestryUniversity,Lin′an311300,China;
3TheSecondMilitaryMedicalUniversity,SchoolofPharmacy,Shanghai200433,China;
4LishuiUniversity,SchoolofEcology,Lishui323000,China)
[Abstract] ThepaperaimstostudytheefectsofendophyticfungifromDoficinalecultivatedonlivingtreesongrowthandcompo
nentsmetabolismoftissuecultureseedlingsMorphologicalcharacteristicsandagronomiccharactersoftissuecultureseedlingsinfected
anduninfectedbyendophyticfunguswereobservedandmeasuredPolysaccharidesandalcoholsolubleextractscontentsweredeter
minedbyphenolsulfuricacidmethodandhotdipmethod,respectivelyMonosacchridecompositionofpolysaccharidesandalcoholsol
ubleextractscomponentswereanalyzedbyprecolumnderivativesHPLCandHPLCmethod,respectivelyItshowedthatefectsof
turningtopurpleofstemnodescouldbechangedbyendophyticfungusBesides,theendophyticfunguscouldafectthecontentsand
constitutionsofpolysaccharidesandalcoholsolubleextractsThestrainstested,expectDO34,couldpromotegrowthandpolysaccha
ridescontentoftissuecultureseedlingsThestrainstested,expectDO12,couldpromotetheaccumulationofmannoseFurthermore,
DO18,DO19andDO120couldincreasealcoholsolubleextractsOnthebasis,foursuperiorstrainswereselectedformechanismre
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searchbetweenendophyticfungusandtheirhostsandmicrobiologyengineering
[Keywords] Dendrobiumoficinale;endophyticfungi;growthanddevelopment;componentsmetabolism
doi:10.4268/cjcmm20160906
  
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ridesandalcoholsolubleextractsfromtissuecultureseedlings
(珋x±s,n=3) %
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cultureseedlingsinoculateddiferentendophyticfungi
Β Š‡c ABc %ùc Ac ²fc
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cultureseedlingstested(B)
·5061·
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Vol41,No.9 May,2016
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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21
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DO18 11 - 10571415 - - 193258742572 - 294 90 - - - - - - 6961244297
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DO34 3 - 891 - - - - - 790366 - - - - - - - - - - - -
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DO120 11 - - - - - 477 - 94312031173249 - 4222291346811 - 1965421 - -
CK 1 - - - - - - - - - - 208 - - - - - - - - - -
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stemoftissuecultureseedlingstested
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