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Rapid analysis of compounds in leaves of Chinese seabuckthorn and Tibetan seabuckthorn by UPLC/Q-TOF-MS

中国沙棘和西藏沙棘叶中化学成分的UPLC/Q-TOF-MS快速分析



全 文 :2016
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RapidanalysisofcompoundsinleavesofChineseseabuckthornand
TibetanseabuckthornbyUPLC/QTOFMS
QINZhenxian1,ZHANGYu1,QIMengdie1,ZHANGQuan1,LIUShuang1,LIMinghui2,LIUYonggang1,LIUYong1
(1.BeijingUniversityofChineseMedicine,Beijing100102,China;
2.BaotouMedicalColege,Baotou014060,China)
[Abstract] ThestudyisaimedtoanalyzethechemicalcomponentsinleavesofChineseseabuckthornandTibetanseabuckthorn
qualitativelyandcomparethediferencesbetweenthembyusingultraperformanceliquidchromatographycoupledwithquadrupoletime
offlightmassspectrometry(UPLC/QTOFMS)ThechromatographicseparationofthecomponentswasachievedonaWatersACQU
ITYUPLCT3column(21mm×100mm,17μm)usinggradientmobilephaseconsistingofacetonitrile(A)andaqueoussolution
(B)Theidentificationoftheseparatedcompoundswasperformedonatandemmassspectrometry(MS/MS)byfragmentationpaterns
underthenegativeelectrosprayionizationTheparametersofionsourcewereasfolows:capilaryvoltage,2000V;Conevoltage,40
VTheionsourcetemperature,100℃;colisiongasargon;sheathgasflowrate,900L·h-1;sheathgastemperature,450℃
Throughtheanalysisofmassspectrometrydataandwiththehelpofliteraturedata,atotalof35compoundsweredetectedandmostof
themwereflavonoidsAmongthesecompounds,29werecommoncomponentsforthetwospecies,twocomponentswereuniquetoChi
neseseabuckthornand4werecharacteristiccomponentsofTibetanseabuckthornTheresultsindicatedthatthecompositionsofthetwo
kindsofseabuckthornleaveswerequitesimilarItisalsodemonstratedthatUPLC/QTOFMSmethodcouldbeappliedtorapidlyand
efectivelyanalyzeandspeculatethecompoundsinleavesofChineseseabuckthornandTibetanseabuckthorn
[Keywords] UPLC/QTOFMS;Chineseseabuckthorn;Tibetanseabuckthorn;flavonoids
doi:10.4268/cjcmm20160816
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Vol41,No.8 April,2016
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Table1 AnalysisofcompoundsinextractfromtheleavesofChineseseabuckthornandTibetanseabuckthornbyUPLCQTOFMS
No tR/min _HɁ µ-! [M-H]- MS/MSˆ€ª-ví
1 1484
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C34H24O22 783 481,437,301,257,229,185
2 1711
3 2363
4 3028
Nœ-Ä×

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C27H22O18 6330731 463,301,257,229,185
5 3212
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C41H28O26 9350815 891,783,633,481,301
6 3538
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C21H20O11 4471493 301,271,179,151
7 3772
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C33H40O21 7712001 625,447,301
8 4033
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C33H40O20 7552098 593,447,301
9 4235
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C33H40O20 7552026 609,431,285
10 4310
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C21H20O12 4630504 301
11 4596
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C34H42O21 7852144 639,461,460,315
12 4706
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C41H28O26 9350814 891,783,633,481,301
13a 5067 §ìF3O·ßÆÇø™ C27H30O17 6251410 463,317
14 5226
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C27H30O16 6091472 463,447,446,301,299
15 5643
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C20H18O11 4330400 301
16 5863
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C33H40O19 7392099 593,285
17 5973
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C41H53O27 9772586 831,771,625,463,446,301,299
18 6122
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C15H10O7 3009969 257,229,185
19 6242
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C27H30O16 6091415 301,300
20 6454
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C28H32O16 6231609 477,461,460,315,313
21 6511
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C21H20O12 4630869 301
22 6635
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C42H55O27 9912736 845,785,639,461,460,315,313
23a 6772 Û·ßF3O}9ø7O·ßø™ C27H30O15 5931515 461,460,447,315,313
24 7378
y¼Æ
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ÆÇø·ßø™
C27H30O15 5931508 285,227,159,117
25 7555
ºsF
3O
·ßø™
C21H20O11 4470553 301,300
26 7676
y¼Æ
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C21H20O11 4470912 285,284
27 7728
Û·ßF
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Wèø™
C28H32O16 6231611 315,300,271,151,107
28 8068
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ÆÇø™
C22H22O12 4771027 315,300,271,151,107
29 9798
y¼Æ
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ÆÇø·ßø™
C27H30O15 5931314 285,227,159,117
30 10148
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C27H30O15 5931309 285
31 10198
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C27H30O15 5931295 285
32b 3329 ºsF3OWèø7OÆÇø™ C33H40O21 7712000 609,463,301,299
33b 5094 ºsF3OÆÇø·ßø7O·ßø™ C33H40O20 7552054 609,463,446,301,300
34b 6695 y¼Æ3OÃøOÆÇøøç7O·ßø™ C39H48O26 9312545 785,609,285,284
35b 6801 Û·ßF3OÃøOÆÇøøç7O·ßø™ C40H50O27 9612642 815,785,461,315
  
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