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作 者 ：缪成贵,杨剑婷,何华奇,马世堂,周国梁,高敏,刘健

期 刊 ：中国中药杂志 2013年 38卷 12期 页码：1977.

关键词：白头翁皂苷；类风湿性关节炎；SFRP2；Wnt通路；β-catenin；C-myc；

Keywords:pulchinenoside, rheumatoid arthritis, SFRP2, Wnt pathway, β-catenin, C-myc,

摘 要 ：目的: 研究中药白头翁主要活性成分白头翁皂苷(pulchinenoside,PULC)对类风湿性关节炎(rheumatoid arthritis, RA)模型大鼠成纤维细胞样滑膜细胞(fibroblast-like synoviocytes,FLS)SFRP2表达的调控. 方法: 采用大鼠关节炎评分法和足爪肿胀评分法评价PULC对RA模型大鼠的治疗作用,MTT法检测PULC灌胃治疗后对FLS增殖抑制作用.RT-PCR,Western blotting检测PULC灌胃治疗对FLS SFRP2基因表达的调控作用,对Wnt通路关键基因β-catenin,Wnt通路下游效应基因C-myc的调控作用. 结果: RA模型大鼠FLS中SFRP2基因表达明显下调,经PULC灌胃治疗后,RA模型大鼠FLS中SFRP2表达上调,β-catenin和C-myc基因表达明显下调. 结论: PULC通过对Wnt通路的调控抑制RA模型大鼠滑膜异常增殖.

Abstract:Objective: To study the effect of pulchinenoside (PULC) in modulating SFRP2 expression in fibroblast-like synoviocytes (FLS) of rheumatoid arthritis (RA) model rats. Method: The effect of PULC in treating RA rats was evaluated by rat arthritis score and paw swelling score. The inhibitory effect of PULC on FLS proliferation was detected by MTT reagent. The effects of PULC gavage treatment in modulating gene expression of FLS SFRP2, critical gene β-catenin of Wnt pathway and downstream effector genes C-myc of of Wnt pathway were detected by RT-PCR and Western blotting. Result: PULC had a significant effect in treating RA rats and that SFRP2 expression was down-regulated in FLS. After PULC gavage treatment, FLS SFRP2 expression was obviously up-regulated, whereas β-catenin and C-myc gene expressions were significantly down-regulated. Conclusion: PULC can inhibit abnormal proliferation of synovial membrane by modulating Wnt pathway of RA rats.

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