作 者 :杨全,唐晓敏,潘海运,梅凌峰,张春荣,程轩轩,黄璐琦
期 刊 :中国中药杂志 2015年 40卷 20期 页码:3953.
关键词:广金钱草 ;种子劣变 ;贮藏时间 ;生理生化 ;
Keywords:Desmodium styracifolium , seed deterioration , storage time , physiological and biochemical changes ,
摘 要 :以广金钱草种子为材料,研究4℃贮藏条件下不同贮藏时间种子的发芽率、发芽势、千粒重、抗氧化物酶活性及种子浸出液电导率、可溶性糖、可溶性蛋白、淀粉含量的变化,以揭示种子劣变机制。结果表明:①随贮藏时间的延长广金钱草种子发芽率、发芽势、千粒重逐渐降低,种子颜色逐渐加深。②在种子发生劣变的过程中,SOD,POD活性变化趋势类似,均随贮藏时间的延长而降低。SOD活性在贮藏1095~1185 d降低幅度最大,POD活性在贮藏365~395 d显著降低。③种子浸出液的电导率、可溶性糖含量、淀粉含量随贮藏时间增加而增加,可溶性蛋白含量呈降低趋势。④对各项特征指标进行相关分析发现,广金钱草种子发芽率、发芽势、千粒重与SOD,POD活性呈显著或极显著正相关关系,与种子浸出液电导率、可溶性糖含量、淀粉含量呈极显著负相关关系。研究发现,在广金钱草种子贮藏过程中,种子抗氧化酶活性降低、浸出液电导率上升、可溶性糖和淀粉外渗及可溶性蛋白的降解等均是导致种子发生劣变的主要原因。
Abstract:The dynamic changes of germination percentage, germination potential, thousand-seed weight, antioxidase activity in Desmodium styracifolium seeds with different storage time were tested, and electrical conductivity, contents of soluble sugar, soluble protein, starch in seed leach liquor were also determined in order to reveal the mechanism of seed deterioration. The results as the following. ①The germination percentage, germination potential and thousand-seed weight of D. styracifolium seeds declined, while the seed coat color darkened with the extension of storage time. ②The activities of superoxide dismutase(SOD) and peroxidase(POD) decreased with the prolongation of storage period. The SOD activity declined fastest in 1095-1185 d of storage, while the POD activity declined significantly in 365-395 d of storage. ③ The electrical conductivity and the contents of soluble sugar, starch in seed leach liquor increased, while the content of soluble protein declined with the extension of storage time. ④ Correlation analysis indicated that the germination percentage, germination potential and thousand-seed weight of D. styracifolium seeds have a significantly positive correlation with SOD and POD activity, while have a significantly negative correlation with the electrical conductivity, contents of soluble sugar and starch. It can be concluded that during the storage of D. styracifolium seeds, physiological and biochemical changes including decrease in antioxidase activity, rise in electrical conductivity, degradation effluent of soluble sugar and starch, degradation of soluble protein were the main factors leading to the seed deterioration.
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