免费文献传递   相关文献

Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles

基于双向位点特异性PCR的金银花真伪鉴别方法研究



全 文 :ÓÔíîïðñò§
D<>
‡Áóô
\õ­ìö÷ˆ‰

#
9‘D
#

#
“Ž
!
#
u”A
#
M•–
!
ê]êõåºé êãìíî
#

$$%$$

Z[
#!
Pz
#
¨©…dÁóô\õ­ì
8;D
ïð¬Àbíîïðñò§
D<>
ö÷
$
Ô­ìÁóôé8ªtõ:‰
ª:܇9-:

{|
#
BÇý
FO1SI1X
R÷‡ñÐä€{_óh
)*W4)*d
};Šfýú«O
$
…dÁóô\õ­ì
8;D
ïð
)
¬\‚C
8;D
ïð†{ñò§g{
$
ý
(&
äÁóôÓ¸€{ª84°òd
(
õ:Šfíîïðñò§
D<>

$
¬’
‚\õ:‡ñò§¼ÃŠfÁóô,¤­ì

}~
#
܅ŸWŒ
# f
T
$
`:½´w
&#( \Y
‡¼Ã
$
Ø«ñÐ
(
_9ñÐ
(
S
óYñÐ
(
6§YñÐ
(
øñÐ
(
ÁóñÐ
(
­WñÐ
(
¶·ñÐ
(
ô„ñЋ
7
òõ:½´w
)*& \Y
‡¼Ã
$
`:
T;3

1
j
‰²õ:TPT´w`:éõ:¼Ã

}V
#
íîïðñò§
D<>
ˆ‰­ìÁóô\õ:ª:܇9-_:


\]^
#!
íîïðñò§
D<>)
Áóô
)
«ë­‘

_`-F
#!*$*$#$#$$#

abcP
#!
%€ôÃ&ÓÁI¸
!($$#$)
$,™,Çȶ
()%€yðmNÍ#ØÓÁI¸
!8aW*$$9$$

dXef
#!
!
DE
$
€ˆ‰r
$A4/IKQ#55RI1$%)*2H/IKQc-./
!!
ÁóôŒñÐ&€{ñÐ
J,*1:-)& >&4,*1:&
BJR1\c
‡^ÔôõÛÃb#‡ô
$
¹z¦§Þ«Œ
ó¸2
(
³ö=\‹6ä¬{É

Áóô‚
Þª=ï
$%
$%,P
&
Þöé™>$,$ñG
#
òö>‰Áóôƌ¹z‡ÊÞÞ«
$
wU,m
NV©8xG&ž
(
Ÿ#
(
Ÿ(Ž
(
ŸÊD
(
îå
(
í
¾
(
6Êêë
(
ª¿
(
$÷žøéuAª«
(
ŸXÌD
´‹Æ
.4/

Cf
*$$
qO
%
$%,P
&
.#/
$0©U}ñ
ÐƌÁóôùÁ€{Ù@
$
¼ÀqpO
%
$%,
P
&
’‚‚îD
(
KO,úû‹
$
®TF©‘ñ
Ð
(
Ø«ñÐ
Jc(D4,0;&+:&(
_9ñÐ
Jc:,*<+!&(
ø
ñÐ
Jc=&!D!D;&
‹ŒÁóô‡Ó¸€{

q
*$$
qO
%
$%,P
&
PTÿ‘Ø«ñÐ
(
_9ñÐ
(
S
óYñÐ
Jc3&:)&*(,1=-!
é6§YñÐ
Jc<+;?,,2
3-*,!&
;ŒÌóô,¤Ó¸€{

ÖX
$
“ԐP
KO½GÁóôú:
$
ɳ42²yB‡Áóô
Á6Þª9ü

λV,`o
$
³X³ÇA÷ö÷ýÁó
ôŠf\m§­‘
$
ÃÈÁóôª8õ:‡çBé
DÞ«ÕWM¼
$
qEœçBéD­ìªŒ
³ŒÛRž•–‡“”

ÄÀMú
$
«ëÚە
xGÁ‘
(
Œ²ûéRž•–“”‡ñð
$
åÔ
Áóô‡«ë­ì

¼¸q0cL‡Áóô«ë­
ìö÷ßߺwŒyw§µ
!
ø
:88>
.%/
(
_:Ö
˜
!
ø
>dWD
.(/
(
¾xU
!
ø
T;3\IN-.PK1H
.7/
(
”
Æ#-
!
ø
88>
.$/

‹D|
$
èޛmh9z
$
•
Ö%9ÀbÁ‘
(
ý=
(
B§ä‡«ë­ìö÷

íîïðñò§
D<>!\KPKNO-LK.1IQD<>I/YQK4
MK-ILK.1 .MCYO-KMK-IQOQOC$SK4D383$
ˆÔM×(
òïð‡¹\2æò
$
xGYû§‡ñð

BÇ(
ò
D<>
±vˆ­ì\õ:
$
0ވÔ³´
./
(
â
.*/
(
ýš
.)/
‹$,¤‡\õ­ì

šCBÇ
³íîïðñò§
D<>
()
$
#(ò
8;D
ïð
PÁ
D<>
±$ýÁóôª8
7
òõ:Šf­
‘
$
¬þeý\õ9-:­ìŠfŠ‹
$
ŒÁóôm§­ìžý(–>‡ö÷

!
¤EÄö÷
Z!
€{
!
©*
(&
ä¤E
$
01ÙôÔ
$
òK
‡
%%
ä€{_:
$
8$ñÐ
*7
ò
(
Ø«ñÐ
(
ò
(
_9ñÐ
&
ò
(
SóYñÐ
#
ò
(
6§YñÐ
#
ò
(
øñÐ
(
ò
(
ÁóñÐ
Jc3&&:C1&
ò
(
­WñÐ
Jc
<)&0)&*1!13& &
ò
(
¶·ñÐ
Jc&&)1:& &
ò
(
ô„
ñÐ
Jc&&)1:& -ik<&*20+,k&
ò
)
‰ª
%
kÈô42
²Aǘ™J‡,¤Šfíîïðñò§
D<>
€{_:¹z(ô=P
(
ç9
(
Ì5
(
½
(
ÿ9
(
2
3
(
`aKO
$
-VښªÔ$%$¦&º$,
ˆ‰8é=P,€{P
$


**%)*
v
)%
pv
*&
x
*$*
q
*
r
!!!!!! ! ! ! ! ! !!!! ! ! ! !
[.Qc)%$ :CCRO!*&
TO-O/\ON$ *$*
º!
eN¤E
BI\QO!DQI1LCI/YQOC
;.c
/< {÷ K ò? ­‘³
W?*$wd` J,*1:-)& >&4,*1:&
ç9„“
&
ÀØù
* W?*$wWG Jc>&4,*1:&
Ì5!
#
­¨
) W?*$w9^ Jc>&4,*1:&
ç9¶%
&
ÀØù
& W?*$w;; Jc>&4,*1:&
=P9¸
&
ή_
W?*$wWGF Jc>&4,*1:&
½`-Ã
&
¥V
# W?*$*wG@ Jc>&4,*1:&
ÿ9í
&
´_Í
% W?*$wVd Jc>&4,*1:&
`a@#

´_Í
( W?*$wFW Jc>&4,*1:&
=P$Ë
*
|Ñÿ
7 WV*$w;; Jc(D4,0;&+:&
=P¢Ý
&
ή_
$ WV*$w;; Jc(D4,0;&+:&
=P$Ë
&
ή_
W<*$wFW Jc:,*<+!&
=P$Ë
)
ή_
* W<*$wWG Jc:,*<+!&
Ì5!

­¨
) W9*$wFW Jc3&:)&*(,1=-!
=P$Ë
*
|Ñÿ
& W9*$w;; Jc3&:)&*(,1=-!
=P9¸
&
ή_
Wd*$wFW Jc<+;?,,3-*,!&
=P$Ë
#
|Ñÿ
# WT*$wFW Jc=&!D!D;&
=P$Ë
&
|Ñÿ
% WT*$w<@ Jc=&!D!D;&
=P¢Ý
&
ή_
( W9I*$*wS? Jc3&&:C1
23
)
´_Í
7 W9I*$wVd Jc3&&:C1
`a@#

´_Í
*$ WdN*$wS? Jc<)&0)&*1!13&
23
&
Ñ¢˜
* WB*$*wS? Jc&&)1:&
23
&
Ñ¢˜
** WB-*$*wS? Jc&&)1:& :?c0MI14HR.1
23
&
Ñ¢˜
*) W?*$w;? Jc>&4,*1:&
93
!

*
Ñ¢˜
*& W?*$wS? Jc>&4,*1:&
23
!


Ñ¢˜
Z!8;D
ïð‡¨©ég{†{
!
#
FO1SI1X
?‚d$ñÐä€{‡_óh
)*d4)*W
};
$

…½Šf};úý
$
«Oƒ„º©ñЇ
)*d4)*W
};
#*
ïŒ
F$
8û{÷½Œ
3
ÓÔ
Ö
8;D
ïð†{íîïðñò§
D<>
g{
$
g{}
;tº
*
º
*!
g{ª
D<>
±¼½
BI\QO*!DNK/ONCI1P NOI-LK.1 -.1PKLK.1C
g{ };
!k4)k D<>
È}
W04d FBBF3ò„R
!7& f )$ C$ # f )$ C$ %* f & C$ %* f % /K1
W04> FF3BF3F333B3B33Q04*d BBB3B<Q04*> LN1W FFBB<33FB<<ò„R
!7& f )$ C$ & f )$ C$ %* f & C$ %* f % /K1
LN1d 3BBBF33!!
»
#3$Fc
Œ¥¦ñò§³Œg1‡}Ä

Z&!
ӕÊm
T;3
‡*
(D<>
<¥¼½‡U‘
!
*
$$ /H
^Ô¤E
$
³
÷*m
T;3
*P_:
T;3$
ÅVW˜âŒ
$ /H*W
b
$
³
Bg{
)*W
é
)*d
Šf
D<>
±‰M×!0
T;3
ÉÖ
D<>
±hiŒ
*c
!
W$ pA68&L
\RMON$*
!
W$ //.Q* W
b
P;BDC$
g{p
$c* Y/.Q$
*)%)*
v
)%
pv
*&
x
*$*
q
*
r
!!!!!! ! ! ! ! ! !!!! ! ! ! !
[.Qc)%$ :CCRO!*&
TO-O/\ON$ *$*
$c ]A68&L
¥
$
â
$ 1HT;3
!0

±
AY4
YO1P.NM
IJ‡
9ICLON-5-QON
™
D<>
<¥²Šf
$
±È}tº
*
±ƒwF
D<>
±hi8¦
1

!
W# pQ.IPK1H\RMON$
9ÔFÔ
AS
3+‡
j
¬ÂãˆÁZ¿M×
$ 8G;FA;A
ˆÁÞ2iœ
!FA;A
IJ

‡z
(
Þ2

#ñÐ
8;D
­ìg{
W04d
é
W04>
Šfï
ðñò§
D<>
±
$
¬«ì‘z܅ŸW
(D<>
„
R?
(T;3
!0Ö
(
g{VW
(P;BD

(8&L
¥
÷¬
.N8<3
E@¥
!BIXINI
˜`z{GHI
J
(A68<3
E@¥
!BIXINI
˜`z{GHI
J
(8&LYQRCT;3
E@¥
!
ztz{
!
²M

GHI
J
(8<3
E@¥
!dON/O1LIC
GHIJ
/(8&L
¥Ö
(
P
D<>

.7%$$
™
D<>

!3S:
IJ
(
DB<4$$
™
D<>

!9?>OCOIN-J
u6
(9ICLON-5-QON
™
D<>

!AYYO1P.NM
IJ
(B<4*
™
D<>

!BAIJ
/
ý
D<>
±Á‘§‡“”

‚¶~܅ŸW†{9Û:‡Ó•™­ìg{
Q04
*d
é
Q04*>$

*
Z(!
íîïðñò§
D<>
­ì
!
*P_:
T;3
Šfíî‹ïӕñò§
D<>$ D<>
±
hiŒ
*c
!
W$ pA68&L\RMON$*
!
W$ //.Q
*W
b
P;BDC$
g{
W04d$Q04*>
p
$c$( Y/.Q$
g
{
W04>$Q04*d
p
$c* Y/.Q$$c ]A68&L
¥
$
â
$ 1HT;3
!0

±
AYYO1P.NM
IJ‡
9IC4
LON-5-QON
™
D<>
<¥²Šf

±È}tº
*
±ƒwF
D<>
±hi8¦1

!
W# p
Q.IPK1H\RMON$
9ÔFÔ
AS
3+‡
j
VW¬Â
ãˆÁZ¿M×
$8G;FA;A
ˆÁÞ2iœ‡z
(
Þ2

ñÐ
T;3
*O8¦19Û:‡
T;3$
M
«9@³d9Û:
T;3
\s`m
T;3
Lև
j$j$$j$*$j$$j$7$j$$$j D<>
±
hiª±È}P²

!
ƒ„ªÚc
Z!
íîïðñò§
D<>
g{†{
!

FO1\I1X
?‚d$Y…dñÐä€{_óhé
:B8
};
$$*
¼
$
«äÔ
#
òdS
$
#z{nŒ«OY
¨©´
&
òñÐñG
8;D
ïð

#ïðñò§
D<>
ö÷ý
&
ò
8;D
ïðŠfNV
$
ƒ„º©éG
)*W4)*d#*
ï
<=3
ïðåUZŒñÐ
8;D
­ì
ïð
$
Ԇ{g{Šfíîïðñò§
D<>
íîïðñò§
D<>
.&/
ö,rg{<¥Œ
’næhi
D<>$
8¹z’“Ô
8<3
E@¥

)k4k
Xw¥p`¦§
$
pg{
)k
µ}ÄT
àÞ
D<>
(
<¥Œ’
$
•ÖˆBÇ
†{ñò§g{ý
8;D
ïð‡
*
ò‹ïӕŠ
f
D<>

$
xhŽti

Ayïðñò§
D<>
Bª9z
*
ò‰²ïðÙO«
*
÷ӕ™
$
Ä8Mú
$
íîïðñò§
D<>
é9zÁòï
ð
$
qÁs
D<>
±$vˆO«
*
÷ӕ™
$
$,­‘$xGÒä‡q}

i
!SK4D383
Áóô\õ­ìö÷á{i
dKHc ! BJOLJO.N5.MSK4D383 M.NIRLJO1LK-ILK.1 .MdQ.C
W.1K-ONIO?IY.NK-I
Z!D<>
±¼½‡U‘
!
Œ…d¶¾‡
D<>
±¼½
$
šCQTÀbž(úïðñò§
D<>
!I/YQKMK-ILK.1 NOMNI-L.N5/RLILK.1 C5CLO/$ 3>98
ö÷
$
¬ý8±¼½Šf¾D
$
ƒ„tº
)

Öӆ²†{¹Áúg{
$
¬ý±„R?ª8
Xúg{VWúŠf¾D
$
‰U‘
SK4D383
‡±
¼½

mNƒ„º©
$
܅ŸW
(8&L
¥÷¬ª
Öý
3>98
«™“”G˜
$
qÁóô
3>98
ª
SK4D383
«™‡
D<>
¼½$!0
(
g{
(8&L
¥‡
³Ö½n]Õ
$
ØÄq³cLM%
./

“
Ô
SK4D383
³(°g{
$
ˆè+àÞ`ª
D<>
<¥Œ&™‡ýþ
$
!&‹
.#4%/
¾D
SK4D383
T

q
Û
*q
‡8Xúg{VWúƒ„G¾
$
šC«Oƒ„º©8Xg{‡VWúŒ
)q
T
$
Áóô
SK4D383
ƒ„Gä

Z&!
íîïðñò§
D<>
­ìÁóôª8õ:
!
#†{‡Bg{éíîñò§g{ý
*7
òñ
Ðòh
(&(
òõ:òhª
%
kP,(ȇÁó
ô,¤
T;3
Šf
D<>

$
ƒ„ti
*
³
)*W4)*d
Bg{½ˆ<¥´
$$ \Y
‡dS
)
³
Áóôïðñò§
D<>
g{
$
éGÁóô_:è
<¥´
&#( \Y
‡dS
$
ȳÁóôõ:ïðñò
*&%)*
v
)%
pv
*&
x
*$*
q
*
r
!!!!!! ! ! ! ! ! !!!! ! ! ! !
[.Qc)%$ :CCRO!*&
TO-O/\ON$ *$*
!!
º
)!
P
D<>
±¼½ýÁóô
3>98
‡“”
BI\QO)!3/YQKMKOP NOCRQLC.MPKMONO1LK1MQRO1-OMI-L.NCK1 3>98c
•– ¼½
W? WV W9I- W< WdR WT W9II WdN WB WB-
„Rs?
)$
$
b b b b b b b b b
)*
$
b b b b b b b b b
)) h b b b b b b b b b
)& h
$
b b b b
$
b
$ $
8
/
=f
#$ h h
$
b b b b b b
$
# h b b b b b b b b b
#*
$
b b b b b b b b
$
8&L
¥Ö
=] $c* h b b b b b b b b b
$c$ h b b b b b b b b b
c$$ h
$ $
b b b
$ $ $ $
c h h h
$
b b
$ $
b b
g{VW
=Y/.Q
$c*
$
b b b b b b b b b
$c* h b b b b b b b b b
$c h h h b b b b b b b
T;3=1H
$
b b b b b b b b b
)
$
b b b b b b b b b
$ h b b b b b b b b b
)$ h b b b b b b b b
$
7$ b b b b b b
$
b b
$
P;BD=1/.Q b b b b b b b b b b
$
$
b b b b b b b b b
)$ h h
$
b b b b b b b
8&L
¥÷¬
8&LYQRC b b b b b b b b b b
8&L b b b b b b b b b b
A68&L h b b b b b b b b b
N8&L h b b b b b b b b b
²
9ICLON-5-QON h b b b b b b b b b
B<4* b b b b b b b b b
$
7%$$
$
b b b b b b b b b
DB<4$$ h h b b b b b b b b
!!
»
#W?c
ñÐ
) WVc
Ø«ñÐ
) W9I-c
SóYñÐ
) W_9ñÐ
) WdRc
6§YñÐ
) WTc
øñÐ
) W9IIc
ÁóñÐ
) WdNc
­WñÐ
) WBc
¶·ñÐ
) WB-c
ô„ñÐ
) hc
Ô¼Ã
)
$
c
£¼Ã
) bc
‰¼Ã

§
D<>
g{
$
õ:½ˆ<¥´
)*& \Y
‡dS
$
È`
:+<¥´¶Û¼Ã

`±îg{8Êއíî
ïðñò§
D<>
g{
$
ˆPÁ
D<>
±$­ì
`:éõ:

Z(!
íîïðñò§
D<>
­ìÁóô
!
“Ôíî
ïðñò§
D<>
Yû§‡ñð
$
ýÔÁóôª8ô
õ:
$
ˆBÇíîïðñò§
D<>
¼ÃŠfOì

ñÐ$ô1
j
‰²õ:Tvˆê‡M×õ
:¼Ã
$
ti
)
&!

(¹\2AB§
!8;D
Ó•Êøù²=ï

$
•8«b=ï
(
?Ö(A
$
í‹ïñ§lÔM×
$
ªو‰{÷‡)@ĊD
$
Šf{÷‡­‘
$

,¤¢¦÷­ìˆ‰$xGyz{|

ÓÔ
8;D
ӕ«™‡­ìˆ‰$
$
íî‹ïӕñò§
D<>
áBÇ(ò
8;D
ïðvˆO«
*
÷ӕ™
$
xGY
û§
(
lÔMׇñð
$
,¤\õ:–—M×ö¿
xG§ñ‡¾{

šˆ‰Àbžíî‹ïïðñò
§
D<>
ö÷
$
ˆÔ­ìÁóôÙ@€{ñЪ8
_9ñÐ
(
SóYñЋ
7
÷ªõ:
$
qBÇ(s
D<>
vˆ­ìôõšÇ
j
‡9-:
$
ýÔÁóô
+
`š@
,
鍎‡ˆ¬­e‹xGyz{|

*%)*
v
)%
pv
*&
x
*$*
q
*
r
!!!!!! ! ! ! ! ! !!!! ! ! ! !
[.Qc)%$ :CCRO!*&
TO-O/\ON$ *$*
3c
Bg{
) Sc
ñЭìg{
) õ:­ìg{
)Tc
íîïðñò§
D<>
g{
)9cTW* $$$ /INXON) c
ñÐ
) *c
Ø«ñÐ
) )c
SóYñ
Ð
) &c
_9ñÐ
) c
6§YñÐ
) #c
øñÐ
) %c
ÁóñÐ
) (c
­WñÐ
) 7c
¶·ñÐ
) $c
ô„ñÐ
) ;c
ù§ý¡

i
*!
íîïðñò§
D<>
­ìñЪ8õ:ˆÁZ¿
dKHc*!3/YQKMK-ILK.1 .MJ,*1:-)& >&4,*1:& I1P KLCIPRQLONI1LC
9cTW* $$$ /INXON) c
ù§ý¡
) * e%c
«ìŒñÐ$ô-ž
j$
j$$j$ *$j$$j$7$j
‡_9ñÐ
) (c
_9ñÐ

i
)!
PúvñÐ
4
_9ñÐíîïðñò§
D<>
dKHc)!3/YQKMK-ILK.1 NOCRQL.MPKMONO1LYON-O1LIHO/K6LRNO.M
J,*1:-)& >&4,*1:& I1P Jc:,*<+!-

ijWk
#
. /!@JO1H $` GROD$ ER S$ OLIQcDJIN/I-.XK1OLK-C-./YINILKiO
CLRP5.MI1.iOQYILK\KQKL5.?/c?ALJ1.YJIN/I-.Q$*$$)%!#**c
. * /!>5R aV$ >JOOV:$ aK/?V$ OLIQc31LK4K1MQI//IL.N5I1P I14
IQHOCK-I-LKiKLKOC.M8aW?:$IJKHJQ5YRNKMKOP I1P K10O-LI\QOJON\IQ
O6LNI-L.MJ,*1:-)& >&4,*1:& .?/cSK.C-KSK.LO-J1.QSK.-JO/$
*$$$%&!$#*$**c
. ) /!>IJ/I1 3$ aI1H8 CY.KQIHO\I-LONKI\5OCCO1LKIQ.KQI1P OLJI1.QO6LNI-LC.MJ,*1:-)&
>&4,*1:& BJR1\.?/cd..P . & /!ER WcAMO-L.M-JQ.N.HO1K-I-KP .1 I1LK.6KPI1LI-LKiKL5.MdQ.C
W.1K-ONIOO6LNI-LC.?/c?@JO0KI1H]1Ki8-KS$*$$%!7#%)c
. /!WOO?V$ a.E 8$ aK/GV$ OLIQc31LK4K1MQI//IL.N5OMO-L.M
LJOI_RO.RCO6LNI-LMN./J,*1:-)& >&4,*1:& MQ.+ONKCNOQILOP L.K14
JK\KLK.1 .M;d4XIYYISI-LKiILK.1 LJN.RHJ NOPR-K1H:4XIYYISIQ4
YJIPOHNIPILK.1 K1 NILQKiON.?/c:1L?9.Q9OP$*$$$%!#%7c
. # /!
$%,P
c
Á1
.8/c*$$#*$c
. % /!
Ã$+
$
)
$
â·*
$
‹
c
PÁóô÷Ɏ@
:88>
!
EA_§ˆ‰
.?/c
rr˜c
#
ôÃ&O
$*$$7$&#
!##())c
. ( /!DO1H^ $ WKE$ EI1HE$ OLIQc:PO1LKMK-ILK.1 .MJ,*1:-)& >&4,*12
:& \5D<>4>dWDI1P IQOQO4CYO-KMK-PKIH1.CLK-D<>\ICOP .1 CO4
_RO1-OC.MK1LON1IQLNI1C-NK\OP CYI-ONNOHK.1C.?/cDQI1LI9OP$
*$$$%#!#&7%c
. 7 /!8R1 @$ FI.B$ GI.V$ OLIQc:PO1LKMK-ILK.1 .MJ,*1:-)& >&4,*1:&
I1P KLCNOQILOP CYO-KOCRCK1HLJOT;3\IN-.PK1H/OLJ.P.?/c
DQI1LI9OP$*$$%%!)#)$c
.$/!

$
DE
$
Às©
$
‹
c
ÓÔ
A8B488>
‡Áóô«ë­ìö
÷ˆ‰
.?/c
,c
$&%!*#($)c
./!EI1HV$ aK/9a$ a+.1 E 8$ OLIQc9.QO-RQINIRLJO1LK-ILK.1
.M$&*&/01*!-*0 I1P HK1CO1HYN.PR-LCRCK1HN.\RCL8;D/INXONC
K1 NK\.C./IQO6LON1IQLNI1C-NK\OP CYI-ONNOHK.1.?/c?DJIN/SK4
./OP 31IQ$*$$!#7%*c
.*/!EI1HV$ aK/9a$ aK/G?$ OLIQc9.QO-RQINIRLJO1LK-ILK.1 .M
LJO.NKO1LIQ/OPK-K1OCDONK-INYKR/OLK-RQILIOI1P CJKRCDONK-INYKR/RCK1H8;D/INXONC.?/cFO1O$*$*$&7&!#
7*c
.)/!?KHPO1 S$ EI1HV$ a5R/a9$ OLIQc3RLJO1LK-ILK.1 .MLJO.NK4
O1LIQ/OPK-K1IQYQI1LJ10+!1:+3-*+1!13+3!;IXIK aKLIHI+I
*#%)*
v
)%
pv
*&
x
*$*
q
*
r
!!!!!! ! ! ! ! ! !!!! ! ! ! !
[.Qc)%$ :CCRO!*&
TO-O/\ON$ *$*
!a.NOI1 F.4S.1 \5/RQLKYQO6D<>.?/cDQI1LI9OP$*$$$%#
!###&(c
.&/!WKR $`BJ.NQI1P A<$VOKL?3$OLIQcZiONQIYYK1HD<>M.N\KPK4
NO-LK.1IQD<>I/YQKMK-ILK.1 .MCYO-KMK-IQOQOC# INIYKP .1O4LR\O
/OLJ.P M.NCK/RQLI1O.RCQ5PKMONO1LKILK1HJ./.U5H.LOCI1P JOLON.4
U5H.LOC.?/cFO1./O>OC$77%$%!&#)(7c
./!
ÕØ
$
¥.¨
$
!°Ë
$
‹
c
íî‹ïӕñò§
D<>
–—O
«3@ëé-@ë
8;D
«™‡¶ö÷
.?/c
¦«ëz{
-.
$*$$($!##*&c
.#/!
!*
$
ãÁb
$
ÖÆÆ
$
‹
cD<>4Á÷ÓÔ}Ä()‡
8;D
«™ö÷‡óö
.?/c
!E
$*$$))!*#(*c
.%/!
jÿ
$
¡Éö
$
¥·µ
$
‹
c
rg{
D<>
<¥±‡(f
8;D
–—ב÷
.?/c
$%z{DÄ«ëz{c
$*$$&$*$
!*#**c
4GBC?AB.J-B.@A@LA3,6$%( B(#3,6( GI.AS K.F.E?JB.@A-/N08
-,M/.L.J-B.@A@LIM?J.L.J-/?/?I
?:3;F!
$ W:;8JR4MI1H$ E]@JK4HI1H
!@*!1+-,<%(1*-!-I&-)1& I-=1:&$ %(1*& 7:&=-3D,<%(1*-!-I-=1:&;#:1-*:-!$ G-1>1*0 $$%$$$ %(1*&
.4KIBE-JB/!6KV?JB.Q?# B.KPO1LKM58;DK1 MQ.CW.1K-ONIO$ I1P IRLJO1LK-ILOJ,*1:-)& >&4,*1:& MN./KLCIPRQLONI1LCI1P LJO/K64
LRNO\5RCK1H\KPKNO-LK.1IQD<>I/YQKMK-ILK.1 .MCYO-KMK-IQOQOC!SK4D383,&4,*1:& I1P KLCIPRQLONI1LC+ICKPO14
LKMKOP \5RCK1HLJOD<>NOI-LK.1 C5CLO/CK1-QRPK1HI11OIQK1HLO/YONILRNO.YLK/KUOP,ZYLK/KUOP NOCRQL+ICYONM.N/OP K1 (& CI/YQOCL.IRLJO1LK-ILO
JK>&4,*1:&$ KLCIPRQLONI1LCI1P LJO/K6LRNO,8?IG/B#EJO1 LJOI11OIQK1HLO/YONILRNO+IC# f$T;3MN./JK>&4,*1:& +.RQP \OI/4
YQKMKOP &#( \Y +JONOICD<>YN.PR-LCMN./IQ.MLJO7 IPRQLONI1LC+ONO)*& \Y,BJOOCLI\QKCJOP /OLJ.P IQC.-I1 POLO-Lj .MK1LO14
LK.1IQIPRQLONILK.1 T;3K1L.JK>&4,*1:&,0@AJ/GI.@A# BJOSK48D383-.RQP IRLJO1LK-ILOJK>&4,*1:& MN./KLCIPRQLONI1LCI1P LJO/K64
LRNO,
.\?D T@EFI/!\KPKNO-LK.1IQD<>I/YQKMK-ILK.1 .MCYO-KMK-IQOQOC) J,*1:-)& >&4,*1:&) /.QO-RQINIRLJO1LK-ILK.1
F@.#)](^_PJVJ,,)($

lm-0
!
pqr
#
*%%)*
v
)%
pv
*&
x
*$*
q
*
r
!!!!!! ! ! ! ! ! !!!! ! ! ! !
[.Qc)%$ :CCRO!*&
TO-O/\ON$ *$*