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作 者 ：廖红波,刘屏,胡园,王东晓,林辉

期 刊 ：中国中药杂志 2009年 34卷 12期 页码：1562.

关键词：胡椒碱；SH-SY5Y；悬尾；强迫游泳；抗抑郁；神经前体细胞；

Keywords:piperine, antidepressant, SH-SY5Y, neural progenitor cells,

摘 要 ：目的：观察胡椒碱的抗抑郁及细胞保护作用。方法：利用小鼠强制游泳实验、悬尾实验考察胡椒碱抗抑郁作用；采用皮质酮损伤SH-SY5Y神经细胞和分离培养新生大鼠海马神经前体细胞两种体外模型，测定细胞存活率（MTT法），观察胡椒碱对神经细胞增殖的影响，并初步探讨其抗抑郁的作用机制。结果：胡椒碱(10，20 mg·kg-1)给药后，能明显缩短小鼠在行为学实验中的不动状态时间，并且该作用随剂量增大效应逐渐降低；胡椒碱（1.4，7，35 μmol·L-1）可对抗皮质酮对SH-SY5Y的损伤作用，且对所培养具有神经前体细胞特性的细胞，具有促进神经前体细胞增殖的作用。结论：胡椒碱具有较好的抗抑郁作用，其作用可能是通过调控神经干细胞的增殖、迁移和分化及对功能性神经再生的保护和促进作用实现的。

Abstract:Objective： To observe the antidepressant effect of piperine and its neuroprotective mechinism. Method： The behavioral studies were performed in forced swimming test (FST) and tail suspension test (TST). To further explore the mechanisms underlying their antidepressant-like activities， CORT-induced neuroblastoma SH-SY5Y cells and isolated and cultured neural progenitor cells. By using MTT assay， the effect of piperine on neural cells proliferation was observed. Result： The research results indicated that after a week of administration， piperine (10， 20 mg·kg-1) could significantly reduce the duration of immobility in both FST and TST. Piperine has the protective effect on neuroblastoma cells and increased proliferation of hippocampus neural progenitor cells. Conclusion： In the present study， we demonstrated that the antidepressant-like effects of piperine and its mechanisms might be involved by up-regulation of the progenitor cell proliferation of hippocampus and cytoprotective activity.

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