作 者 :石学魁,阮殿清,王亚贤,马莉,李明琦
期 刊 :中国中药杂志 2010年 35卷 2期 页码:215.
关键词:红花多糖 ;抗肿瘤活性 ;NK细胞 ;CTL细胞 ;
Keywords:safflower polysaccharide(SPS) , anti-tumor activity , cytotoxicity of NK cell , cytotoxicity of CTL cell ,
摘 要 :目的: 研究红花多糖(safflower polysaccharide,SPS)体内外的抗肿瘤活性及对荷瘤鼠CTL,NK细胞杀伤活性的影响。 方法 :利用昆明种小鼠(KM)制备小鼠肉瘤S180的体内移植性肿瘤模型,腹腔连续给予SPS 10 d,称肿瘤质量;利用T739小鼠制备小鼠肺腺癌LA795的体内移植性肿瘤模型,腹腔连续给予SPS 10 d,称肿瘤质量,测定CTL及NK细胞杀伤活性;用台盼蓝拒染法观察SPS对3种肿瘤细胞的体外抗肿瘤作用。 结果 :SPS 40 mg·kg-1 对小鼠肉瘤S180的抑制作用最明显,抑瘤率为51.33%(P< 0.01);对小鼠肺癌LA795也有抑制作用,使肿瘤体积明显减小为3.29 mm3 (P< 0.05);能明显提高荷瘤小鼠脾CTL细胞、NK细胞杀伤活性(P< 0.05)。 结论 :SPS具有抗肿瘤作用,其作用机制可能与增强CTL,NK细胞的细胞毒活性有关。
Abstract:Objective : To study the anti-tumor activity of SPS in vivo and in vitro and the cytotoxicity of CTL cells,NK cells of T739 lung cancer in mice. Method : The transplanted tumor model of S180 Sarcoma was established with KM mouse. The SPS was adminished ip for 10 d, the tumor weight was detected. The transplanted tumor model of LA795 lung cancer was established with T739 mouse and SPS was adminished ip for 10 d and the tumor weight and the cytotoxicity of CTL cells, NK cells were detected. The Anti-tumor activity of SPS on three types of tumor cells in vitro was observed with trypan blue exclusion staining. Result : SPS 40 mg·kg-1 can significantly inhibit the growth of S180 Sarcoma in mice and inhibitory rate was 51.33%(P< 0.01). It can also inhibit the growth of LA795 lung cancer in mice and the tumor volume was reduced obviously for 3.29 mm3 (P< 0.05). It can remarkably enhance the cytotoxicity of splenic CTL cells,NK cells in tumor-bearing(P< 0.05). Conclusion : SPS have anti-tumor effects, the mechanism of the anti-tumor activity may be related to enhance the cytotoxicity of CTL cell and NK cell.
全 文 :ÖãX»QR(« O%)* YZG
;P [\]E(
8×Ø $ ÙÚÛ + $ OËÁ ) ! $ §Ê ) $ Mu & !K FGL¹×» H<¶Í×Þ½ä $ I;L FGL %$* +K J^þKHLMNÌÂð $ I;L OP¨ *$$* )K I;L¹¼³× Q¶ÍÎRS×Þ½ä $ I;L OP¨ $$&$* &K OP¨¹º³× TUVW¹» $ I;L OP¨ $$( + | ,! Q # /0ÖãX !JBUW/,SCA/W6JB..?BCTVS$8E8 ê=×»QR(«ÑRG[\]E(
) # ë¶?G !P9 Ê>?G:R 8($ ê=2QR±Ç $ ¥r5s 8E8 $ V$ ÊQRª * O%)* ? GÊ>?GYJZ L4%* ê=2QR±Ç $ ¥r5s 8E8 $ V$ ÊQRª $ ÚÛ« ;P [\]E( * + T,(Ùz{ 8E8 ) QR[\ê×»QR? ) #8E8 &$ 0D%\D e ?G:R 8($ WÊ?7¶ $ WRHD K))i!)h$K$* ?GYZ L4%* JWÊ? $ 4QRê{¶{?D )K+* 00 ) !)h$K$* ¶ÂúÑR?G.[&;P [\]E( !)h$K$) #8E8 J»QR? $ 6?´ÊtST[\[\A(J ) + ,! ÖãX * »QR( *;P [*[+ y)( ,!+$$*5$5$# + Q ,! ÿËM9Þß !F+$$%5&$ + f ,! ! ! $ MN $ º»p $OSW#!$&(+*)#&*$G e0BTW#,B2D670BTW36B?//-./0-.2 + ,! íy $ MN $ ç«p$,(^G¾ & »QR & UV 5[/0 $ yDÿ$-,F$S-
¿#º»/0 $ OSW#!$&)#(+#5))()$G50BTW#^J#($+3cc-./0 !! ÖãD)&ãn2ðÖã 1&(-&5$0(#,I (2$0LK ³ÐyÝã $ +HØ(^G¾ù$, $ J(^EJ & ¾1cÖÑÄ +, ) ÖãG¬¾ 2 $ ç«àáÃî9ù & é¹ & Xé ) 6$Öã X !JBUW/,SCA/W6JB..?BCTVS$8E8 JUV5[Ñ $ SSÒX^}fB $ À»töWÊX ^}f? ++, ) EJ 8E8 ?DðUV5[= ôQRhBK`n ) /0Eü?Gê= 2QR±Ç«ê×[\s $ 8E8 »QR? c/0 $ À½¾å6?´Ê ) ! Y! Åð ! LäÀë¶?G !P9$ 01 $ êª ( k++ D$( V> $ :ÿ$-,F!Å ð$; *O%)* ?G $ 01 $& k V> $ êª ( k++ D$ %Ò*ý-&FQR1 ) !×ío !ó>9rs % V) Y! QR[\w ! ?G:R[\w 8($ :k QR/01 * ?G O tuR[\w _4<5 :* ý-&FUVó8È * z1Z[\w ISLB& Z[\w ISA5+& ?GYZ[\w L4%* í:* ý-&FUVó ) Y&! C= !>E9:5#&$ s !H:ML L M * *N^L !a<8$H:ML LM * +T & S õ !8TD0B LM * {7·J|}Yá ! ) I5OV># ÿc( $$ 0e ! $%&.â 1 ) Y`! @AýB> !+ s[ ! p~Gwø + * ~ ! p RW60AXJ* b[\© r³A ! e-@A_ *L8 ($$$ d@ ! Ú% MGôkó« ` ós. &+& ôs . &+$$ 0L s !) Y$! ÖãXÊ> ! Öã $$$ D$ Ôº» $*$ l Âà & ?$ Â2ï $ ü $ +,ª¾Âà º $ ¿À + ºÂÃdi®+ãÛê{ $ Ò®°¯iÏ+ (i) 3QðÒ jX $ dý$ $ ÒìL Ô¶ $ 2- $ JìLH $ S]¾È? # º $ ðJi¸ª ) jHÒ *i ®°3Q $ 3Qð Ï/[j®° & ®òô ) º $ ³Ð/ËnÖã X !8E8) %+% u ) Hu + w +$$ n o !!!!!! ! ! ! ! !!!!!! ! ! ! ! ]/W-)$ :JJXS!+ YB2XBC6$ +$$ ! lÙ Y! [\A(C ! +T,(Ù # ãQR [\¥j $IB2\J dô ) º $ ô i ?Nþ^ L >E9:#&$ sd$s $) V HM º $ J ) º HM/[\ô! * SõG#Bw 8($$L4%*$ISLB [$ ªlsdô + º/ ô! ) ðÐ[\ð g$ & ñ =0L$ vô 0L ô +& ôs.$s $ ¢%Ò¡¢iÏÖãX !+$+$$$$$ $$$ D%L e sd ) ô +&$&( ?$ $K&i +T( $ ]B,(([\B ) Y! »?G2QRC ! Ã¥r=HM % V :R 8($ ?G $ R¥y?G $ o[Òµ¶· Ã¥r=R[$ ð[jô ) º $ $$$ C% 0T2 e ú; 0T2$ ÐLd $ [Ò[j5«R [\ÎÏD g$ % ñ =0L$ \ôë¶?GÏr &· $ vG$K+ 0L) +& ? /|?G³´ ¬ # < $ v< $ ) ÑR`$ 6ä <s 8E8 $$+$$&$$($$#$ 0D% \D e ) m<ívp¥rbc !@ s, º $ s,ä {D +$ 0L%\D e $ 5 $ V$ ve·°êª $ u e¥y?G $ ØúRê $ ÊRª $ ]xRªpBýW RH ) ª¾ ) º $ n»7E= D &$ 0D%\D e ) RªpB f Rª = êª g$$i WRH f! `e = `g$$i) Ã#Bw?GYZ L4%* [$ Sõ G $>E9:#&$ sdô + º $ ]B $ ¬ + g$ # ñ = 0L [\Éd $ à $K+ 0L Ïr·bc O%)* ?G $ Ñ Rºp³´< ) V/ï6&·ÒTUV º ) X<¥rbc 8E8 &$ 0D%\D e $ ÑR`< ¥rbc[j $ vp º ) z{QR°( $ 37ZÚ QRù !B ýFù !^$ ÿ·Ç Lø]xê{ #Nf$K&Bg^ + ) Y&! ?GËo]E(C ! u $ e¥ y O%)* Ñ L4%* ?G ) [ÒÃG.á $ ôÌÍk $Eü $$ ß[\, $ /cÒ IB2\J d ) OCTJ ìGí<Ö[\/ $ ú;ô ) Ý $ Ê>.tu [\Éd ) Ã#B L4%* [&ISLB [& ISA5+ [\SõG $ ô + º $ 5[\+ g$ # ñ = 0L$ Ò $ 0) I5OV>$ /V[ & ?$ #&$ sd $$$ C%0T2 e ú; $ 0T2$ ô + º $ ªl sdʬ[\Éd $ ]B[\ýcë¡ !.A0$ ñ L4%* [\a + ñ .A0) Ó/¬ g $ ñ =0L$ Òo *# ôkós.$ $ vô $K 0L* ¢%vôÒ¡¢< g$ # ñ =0L .[$ j Wê{D $K+ 0L$ Ä 5 O !$$r$ v}©B ) ñkcô $ ¢%BÒÓì< ! O[m sd $ 7Fì< ! 0/W%L e IO[) s + ? / $ vôÒSõ )$$ 0D$F;4 õ 0D$)$ 0T2 /b [\r³Ar³[\Ú .A0 $ õ·ø]xìH !i)!< .A0e ÒÓì< .A0=! 7Fì< .A0e ÒÓì< .A0 g$$i Y`! ?G ;P [\]E(C !;P [\]E( ÚÛ% ) I5OV> 7·O[\D _4<5 [$ Û ¢(ÚÛ ) Y$! Ø] ! 8E88$K$ Ø]ó¶cØ ] $
$ Lj0 -. $ Àc ( [ $ )h$K$ Dn oJØ]Ád ) &!
&Y! [\A(
! 8E8 oê×QR[ 8($$L4%*$ISLB [\7¢s $ J+T,( Ùz{ $
-¶ $8E8 Ðþ ) QR[\[;]E? ) &Y! ?G2QRWÊ? !8E8 $ k&$ 0D%\D e <³= SÒWR?ST $ ð &$ 0D% \D e = WR?7T *($ 0D%\D e = <WR ?¥¦·E $#$ 0D%\D e$ E ±Ç<ÿj[no $ × &$ 0D%\D e D7J Ä= ! - ) 8E8 ?Gêª[¶
) 8E8 O%)* ?Gê= L4%* QR[\JWÊ? $8E8 &$ 0D%\D e !)K+* j$K% 00 ) $ ±Ç< + !$K(( j+K%( 00 ) , ÿj $ QRê {¶{? !)h$K$) &Y&! ÑR?G[\]E(
! L4%* QR[\ O%)* ?G.tu[\ê× L4%* ]E(¶úô ISWB [\ý ISA5+ [\]E( !)h$K$* BL4%* QR[ \,>`FôÑR<ýÑR<]E( !)h$K$* ÑR/ 8E8 <.tu[\ L4%* ]E( úôÑR<]E( !)h$K$$ - +) &Y`! ÑR?G ;P [\]E(
!O%)* ?GÑR`< ;P [\( +!)(K& j(K&*i, %#+% u ) Hu + w +$$ n o !!!!!! ! ! ! ! !!!!!! ! ! ! ! ]/W-)$ :JJXS!+ YB2XBC6$ +$$ !! - !8E8 8($ QR[\ê=WÊ? ! $ Lj0$#f$ < = =0D%\D e êª =D Rª =D RªpB =i WRH =i ±Ç e +&K#& j+K$# K*(( j$K+ (K j+K&$ e 8E8 $ +)K&$ j+K++ K( j$K #K&( jK% +)K#& +$ +)K$& j+K K)( j$K)*+ + K*# jK&+ )$K &$ ++K*) jK*& $K*#% j$K+)& + &K++ j$K*( K)) ($ +)K$$ j+K K+$$ j$K))& + K+* jK%& )*K## #$ +)K&( j+K(# K#)( j$K&+& %K j+K %K( !! b # ±Ç<ÿ )h$K$$ + )h$K$) - +!L4%* æ O%)* ?Gê=( ! $ Lj0$#f$ < L4%* ISWB ISA5+ ` #K%( j)K) (K)* j&K)& +%K) j#K& ` +! L4%* K& j$K% &K)+ j)K+ #K&) j&K%( 8E8 &$ 0D%\D e %)K) j)K)+ $+ ++K(* jK# +#K%* jK% !! b # ` <ÿ )h$K$* ` + <ÿ + )h$K$) j,>`< +!#+K# jK(#i, ¶EF !)h $K$) ê=bc 8E8 / $;P [\(¶bú $ \Õ,>jk +!#K)+ jK#)i,$ ÑR`< ÿj $ noJØ]Ád !)h$K$) `! ´ /0by $8E8 P9 ?Gê= 8($ :R JWÊ? $ Àû-yD¶ Äp ) pWRHtK $ ñ= <$ $&$ 0D%\D e DW R?7E= ) y× $8E8 O%)* ?Gê= L4%* QR[\JWÊ? $ ÑR/J 8E8 QRê{¶{? ) %¶ 8E8 J» QR( ) Eê×[\sBK 8E8 8($$ L4%* ý ISLB [\;\]E? $ Â. 8E8 ¡+Eü;\[\A?½b:WRÄW $ t+Eü5[´êUVÑb:»QR ÄW ) ´êUVÑQRb & bJÎ p ) o»QRUVl§ $ [\UV9çN $ » R[\UV$ $ ;P [\ý O [\Ø+ë ) D] $ /0Bªåø 8E8 ;P [\ý[\
) ;P [\o´êuãLo´Ê$c4ç«Ñ $ ^_»R?¡;«KÏ} $ »RÍs $ [ QRËo $ [ 9I< KÊ $ ;\]EsEü [\AC]EQR ) J/0! $ ã&$, Ãð $ S
ã#jÈ9 & &S¬ÂúÑR? G ;P [\]E( +)5&, ) /0 O%)* ?Gê = ;P [\(ÚÛ
Â. $8E8 ¬6ª tÑR´ê ;P [\WÊÝÞ $ Âú6[\A( )[\ÑQRËo»âm(/¬Ëo ]EQR[$ XtST]E( $ S ÝîXST?G[\QR[\]E? +, ) /0z{» L4%* ÑR?G O%)* .tu [\ê×s L4%* [\J¶]E( $ 8E8 t¶ST%]E( ) !
Â. $8E8 JtEüÂúÑR? G ;P [\ý[\]E(b:»QRÄ W ) C] $8E8 Jý?D$,XÜH$ã ðW5[=ôQR ) ]× $8E8 Jv[\CÑ $ |ôyFþ ) J 8E8 §UV5[ $ bUVÄWé´ $ ÿS 8E8 ?Où & Oµ & O«kAt´Êé $ íJ.ôã¾á/0 ) + , +,! ªþ-. K $,F8 +9,K ÐP # ÐP&()¥y $*(##**+K ++,! È $ ;¦+ $ ¥ K é K ÖãXU( +Y,K yMU V $*(+!##%K +),! 53F $ úk] $ âub K
ã#jÈ9»QR(/0 +Y,K á,` $+$$($+&!+##K +&,! Ȳ $ çké $ ö $ é K &S»QR(«ÑR? GUVÑ
+Y,K $%òó¾ $+$$#$$!#$%K +,! ÝG $ ¿3¦ $ ýk: K ÝîXo©Z$?« ´Ê!/0 +Y,K 75F` # ÒÓ&y $+$$%$( !#+K %%+% u ) Hu + w +$$ n o !!!!!! ! ! ! ! !!!!!! ! ! ! ! ]/W-)$ :JJXS!+ YB2XBC6$ +$$ 3@A-*AF+?D,QA-O-AC ?IH,II.?\>DJ?.CH,QQK,D-E>!:L: ,@E>II>QA ?@QCA?A?M-Q-AC ?I/<8Q>.H$2]Q>.H?I<%)* .F@R Q,@Q>D-@+-Q> 8I:QXS\XT $ >` 4;FTB2cT2D + $ [4;H_B7TB2 ) ! $ 94LT ) $ L:9T2DcT & !DW/@&(5/#(24>,2C2%23+>$6 >/6,&%12%/3/$ >$6 %$$ 1-#&* +K>$% 12$#(+/1/#(/42W/&0/12#(2% )/;/#(2#$ B&C# *$$$ 1-#&* )KW/@&(5/#(24>,2C2%23+ <55$#2%23+$ B/%2#39 :#;/0(+248&6(2#&%1-#/0/ >/6,#/$ B&C# $$&$$ 1-#&* &D8$52B20@(&%24B&C# >/6,&%:#;/0(+$ B&C# $$($ 1-#& +3KHAD,QA,!5K_>QA-O># O/J@XV6@?SB2@T5@X0/CB.@TdT@6/U8E8 # ;;2B2V # ;(2B2V @?S.6@/@/7T.T@6/UO%)* WX2D.B2.SCT2 0T.SK;>AB?E# O?S@CB2JAWB2@SV @X0/C0/VSW/U8($ 8BC./0B,BJSJ@B^WTJ?SV ,T@? P90/XJS-O?S8E8 ,BJBV5 0T2TJ?SV @U/C$ V$ @?S@X0/C,STD?@,BJVS@S.@SV-O?S@CB2JAWB2@SV @X0/C0/VSW/UL4%* WX2D.B2.SC,BJSJ@B^WTJ?SV ,T@? O%)* 0/XJSB2V 8E8 ,BJBV0T2TJ?SV @U/C$ V B2V @?S@X0/C,STD?@B2V @?S.6@/@/7T.T@6/U@X0/CB.@TdT@6/U8E8 /2 @?CSS@6ASJ/U@X0/C.SWJ# ;(2,BJ/^JSCdSV ,T@? @C6AB2 ^WXSS7.WXJT/2 J@BT2T2DK7>HF.A# 8E8 &$ 0D%\D e .B2 JTD2TUT.B2@W6T2?T^T@@?SDC/,@? /U8($ 8BC./0BT2 0T.SB2V T2?T^T@/C6CB@S,BJK))i!)h$K$-:@.B2 BWJ/T2?T^T@@?SDC/,@? /UL4%* WX2D.B2.SCT2 0T.SB2V @?S@X0/Cd/WX0S,BJCSVX.SV /^dT/XJW6U/C)K+* 00 ) !)h$K$-:@.B2 CS0BC\B^W6S2?B2.S@?S .6@/@/7T.T@6/UJAWS2T.@?SB2@T5@X0/CB.@TdT@60B6^ SCSWB@SV @/S2?B2.S@?S.6@/@/7T.T@6/U+]>C \?DEH,!JBUW/,SCA/W6JB..?BCTVS!8E8* B2@T5@X0/CB.@TdT@6* .6@/@/7T.T@6/U;P.SW* .6@/@/7T.T@6/UE?-# (a`U[NQ_Q++((( + -0 ! ÅÆÆ , %(+% u ) Hu + w +$$ n o !!!!!! ! ! ! ! !!!!!! ! ! ! ! ]/W-)$ :JJXS!+ YB2XBC6$ +$$