目的：观察欧亚旋覆花总黄酮类提取物(FE)对大鼠血管球囊损伤诱导的氧化应激反应的影响。方法：采用主动脉球囊剥脱后血管狭窄大鼠模型，将实验动物分成四组：对照组、模型组、FE组和阳性药物(卡托普利，CAP)组；采用常规HE染色法观察血管的形态学变化以确定最佳给药浓度并对比FE与CAP的疗效，分别采用硫代巴比妥酸(TBA)法测定大鼠血清中丙二醛(MDA)的含量，用黄嘌呤氧化酶法测定大鼠血清中超氧化物歧化酶(SOD)活力，用二氢乙锭(DHE)测定超氧阴离子(O－·2)的水平，用免疫组化和Western blot检测血管组织中的SOD表达变化。结果：FE的有效剂量为50 mg·kg-1·d。在此浓度时，FE可显著降低血管球囊损伤大鼠血清中MDA含量(P<001)，提高血清和血管组织中SOD的活力(P<005)，降低血管组织中O－·2水平。因此，FE对损伤诱导的血管内膜增生具有一定的抑制作用，达到与阳性对照药物相似的疗效。结论：从欧亚旋覆花中提取的FE可通过提高血管系统抗氧化活性而抑制损伤诱导的氧自由基生成，这可能是该药物抑制内皮剥脱诱导的血管内膜增生的机制之一。
Objective: To observe the inhibition of flavonoids extract (FE) from Inula britannica on oxidative stress in rat aorta after balloon injury. Method: The model of vascular intimal hyperplasia was established by balloon injury. The rats were randomly divided into four groups: control, model, FE and captopril (CAP, positive control). The FE group was treated by intragastric administration with FE in dose of 125, 25, 50 mg·kg-1·d-1. The level of malondialdehyde (MDA) and superoxide dismutase (SOD) in serum were detected by thiobarbituric acid (TAB) method and xanthine oxidase method respectively, and superoxide anion (O2-) in vessel was detected by dihydroethidium (DHE) staining. The histochemistry, immunohistochemistry and Western blot were used to observe the changes of appearance and SOD expression in vascular tissues after balloon injury. Result: The best concentration of FE to rats was 50 mg·kg-1·d-1. The neointima thickness in the model group was significantly higher than that in the FE group (50 mg·kg-1·d-1) and control group at 14 days after balloon injury (P<001). The lever of MDA in serum of FE group was decreased (P<001) and SOD was increased (P<005) in both serum and vascular tissues. The level of O－·2 in the drug group was lower than that in the model group. Conclusion: FE can enhance the antioxidation capacities of vessel tissues by suppressing the formation of O2- induced by injury, by which FE inhibites neointima formation after balloon injury in rat.