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作 者 ：安丽凤,刘树民,董杨,唐波,董婉茹

期 刊 ：中国中药杂志 2010年 35卷 15期 页码：2026.

关键词：刺五加；PC12细胞；帕金森病；

Keywords:Acanthopanacis senticosus, PC12 cells, Parkinson′s disease,

摘 要 ：目的: 探讨刺五加有效部对MPP⁺诱导的PC12细胞损伤的保护作用。 方法 :以不同浓度、不同作用时间的MPP⁺作用于PC12细胞,MTT法检测细胞存活率,考察其量效关系和时效关系。用不同浓度的刺五加有效部位处理后,与MPP⁺共同孵育,MTT法检测细胞存活率,流式细胞仪测凋亡率,紫外-可见分光光度计测LDH,NO, NOS, MDA含量,考察刺五加有效部位的保护作用。 结果 :刺五加有效部位终浓度达到200,400 mg·L^-1时,可明显提高PC12细胞存活率,抑制细胞凋亡率,降低LDH, NO, NOS, MDA的含量,有统计学意义(P＜0.05)。 结论 :刺五加有效部位对MPP⁺诱导的PC12细胞损伤具有一定的保护作用,其机制可能是通过减少LDH漏出,降低NO, NOS, MDA的含量,从而抑制细胞凋亡,提高细胞存活率而实现的。

Abstract:The purpose of our research was to evaluate the protective effect of the effective part of Acanthopanacis senticosus (AS) on the damage of PC12 cells induced by MPP⁺, an in vitro cell model for Parkinson′s disease Cell viability and apoptosis of PC12 cells induced by MPP⁺ were assayed by MTT and flow cytometry respectively in the prescence or absence of the effective part of AS The contents of lactate dehydrogenase (LDH), nitric oxide (NO), nitric oxide synthase (NOS) and malondialdehyde (MDA) were determined by UV spectrophotometer Our study showed that the survival rate of PC12 cells was markedly increased while cell apoptosis was decreased in the range of 200 to 400 mg·L^-1 of the effective part of AS The contents of LDH， NO， NOS， MDA were reduced Our experimental results indicated that the effective part of AS had the protective effect on the damage of PC12 cells induced by MPP⁺ The underlying mechanisms might be the combination of reduction of LDH leakage and decreases in the contents of NO, NOS and MDA, and finally prevent the apoptosis in PC12 cells and increase the cell survival rate

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