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期 刊 ：中国中药杂志 2008年 33卷 19期 页码：2249.

关键词：过敏性哮喘；小鼠；地龙；

Keywords:allergic asthma, mice, pheratima,

摘 要 ：目的：以小鼠过敏性哮喘模型，探讨地龙酸性部位对过敏性哮喘的治疗作用，并为进一步精制提供依据。方法：通过卵蛋白(OVA)皮下注射致敏，雾化吸入激发的方法，建立哮喘特异性免疫治疗的小鼠模型。通过检测支气管灌洗液(BALF)中的嗜酸性粒细胞(EOS)计数和ELISA法检测BALF中IgE,IL-4,IL-5,IL-13和IFN-γ，考察模型动物与过敏性哮喘相关的炎症细胞水平和免疫系统的Th1/Th2平衡，及药物对它们的影响。结果：较正常小鼠，过敏性哮喘模型小鼠BALF中的嗜酸性粒细胞(eosinophil, EOS)计数显著升高(P<0.01)，IgE,IL-4,IL-5,IL-13水平显著升高(P<0.01)和IFN-γ显著降低(P<0.01)。地龙酸性部位组(S)和30％乙醇洗脱(S30)组有显著的抑制EOS计数升高(P<0.01)，明显抑制IgE,IL-4,IL-5,IL-13水平升高(P<0.05)，和显著抑制IFN-γ降低的作用(P<0.01)。结论：实验提示抑制EOS的分泌和调整Th1/Th2平衡可能是地龙治疗过敏性哮喘的机制之一，S 30在这上述指标上能够较好体现等剂量S的药效作用。

Abstract:Objective: To explore the effects of acidic fraction of Pheratima extract in an ovalbumin (OVA) induced asthma mouse model, and to provide the experimental evidences for the anti-asthmatic application of Pheratima extract with further purification and development. Method: Mice model of allergic asthma was established through the OVA challenge. To investigate the inflammatory cell level and Th1/Th2 levels as well as the therapeutic effects of acidic fraction from Pheratima extract, cell count of bronchoalveolar lavage fluid (BALF) was performed to evaluate the secretion of eosinophils (EOS) cells, and IgE, IL-4, IL-5, IL-13, IFN-γ levels were detected by ELISA. Result: Compared with control group, the EOS count of BALF in the model group was remarkably increased (P<0.01), and IgE, IL-4, IL-5, IL-13 levels were also increased (P<0.01), while IFN-γ decreased (P<0.01). Acidic fraction from Pheratima (S) extract and its 30% ethanol washed fraction (S30) significantly inhibited the increase of EOS count (P<0.01), decreased the IgE, IL-4, IL-5, IL-13 levels (P<0.05), and inhibited the decrease of IFN-γ level (P<0.01). Conclusion: The results indicate that inhibition of the EOS secretion and balancing of the altered Th1/Th2 levels may be important mechanisms of Pheratima‘s therapeutic effect in asthmatic mice model, and S30 is pharmacologically effective as evaluated with the above mentioned parameters, as a representative fraction of the Pheratima extract.

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